巴氯芬对大鼠新鲜分离DRG细胞异四氢烟酸及蝇蕈醇激活电流的抑制作用

Inhibitory Effects of Baclofen on Isoguvacine- and Muscimol-activated Currents in the Neurons Freshly Isolated from Rat DRG

应用全细胞膜片钳技术，研究巴氯芬（baclofen）对新鲜分离的大鼠DRG神经元的作用。结果发现：GABAB受体选择性激动剂baclofen（10－5和10－4mol／L）对蝇蕈醇（muscimol）（10－5mol／L）和异四氢烟酸（isoguvacine）（5×10－5mol／L）-激活电流有抑制作用，并呈浓度依赖性。并对baclofen抑制GABAA选择性受体激动剂muscimol-和isoguvacine-激活电流反应可能的胞内转导机制进行了讨论。

whole cell patch clamp technique was used to study the action of baclofen on the neurons freshly isolated from rat dorsal root ganglion (DRG). It was found that baclofen (10-5 mol/L and 10-4 mol/L) inhibited muscimol- (10-5 mol/L ) and isoguvacine-(5 ×10- 5 mol/L) -activated currents respectively in a concentrationdependent pattern. The possible intracellular-transduction mechanisms underlying the inhibition of muscimoland isoguvacine-activated currents by baclofen were disruss ed.