流式细胞术检测血小板相关抗体初步研究

Detection of Platelet Associate Antibody by Flowcytometry

用流式细胞术对30例正常人及45例免疫性血小板减少性紫癜患者进行了血小板相关抗体（PAIgG）检测并与ELISA法进行了比较。结果发现患者组血小板相关抗体水平为76．14±22．41，明显高于正常对照组26．96±10．42（P＜0．01）。流式细胞术检测PAIgG敏感性为71．4％，与ELISA法接近（71．1％），准确度83．2％，高于ELISA法，假阳性率17．9％，低于ELISA法。对5例患者观察了激素治疗后PAIgG、血小板计数变化，结果均表现为治疗后PAIgG下降，血小板计数上升。认为流式细胞术是一种快速、简便、灵敏的PAIgG检测法，可用于免疫性血小板减少性紫癜的诊断及疗效观察。

Platelet associate antibody (PAIgG) was detected in 30 healthy subjects and 45 cases of immunothrombocytopenic purpura (ITP) by flowcytometry and ELISA, respectively. The results showed that PAIgG level in ITP patients was 76. 14±22. 41, which was markedly higher than in health controls (26. 96±10. 42, P<0. 01 ). Sensitivity of flowcytometry in detecting PAIgG was 71. 4 %, almost the same as that of ELISA. Accuracy was 83. 2 %, higher than that of ELISA. False positive rate was 17. 9 %, lower than that of ELISA. The changes of PAIgG and platelet count were observed by flowcyometry in 5 cases of ITP patients treated with prednison. The results showed PAIgG level was decreased and platelet count increased in 5 cases.It was suggested that flowcytometry is a quick, simple and sensitive assay for detection of PAIgG and can be used as routine method in diagnosis and observation of therapeutic effects in ITP patients.