脑溢安颗粒剂对出血性中风大鼠CA_1区神经细胞凋亡的影响

EFFECTS OF NAOYI'AN GRANULE ON CA 1 REGIONAL NERVE CELL WITHERING IN RATS WITH HEMORRHAGIC APOPLEXY

根据Ｒｏｓｅｎｂｅｒｇ 法复制脑出血大鼠模型；采用细胞形态学、ＤＮＡ 裂解率、流式细胞仪方法，观察脑出血损伤后，大鼠海马ＣＡ１ 区神经元的形态结构，ＤＮＡ 完整性，神经细胞发生凋亡的数量；结果，实施造模手术的大鼠清醒后，均出现偏瘫等神经系统病态体征。造模７ 天时，脑溢安颗粒剂组（以下简称ＮＹＡ组）ＣＡ１ 区锥体细胞形态基本正常，模型组ＣＡ１ 区仍可见到多量的凋亡锥体细胞，差异具有显著性意义（Ｐ＜ ０．０１）；ＤＮＡ裂解率测定，在１ ｄ、４ ｄ、７ ｄ ３ 个时间点上ＮＹＡ 组ＤＮＡ 裂解率比模型组低１０％ ～１４％ ；流式细胞仪检测，造模后７ ｄ，ＮＹＡ组神经细胞凋亡低于模型组，差异具有显著性意义（Ｐ＜ ０．０５）。本研究提示，ＮＹＡ具有抑制神经细胞ＤＮＡ断裂，减少神经细胞凋亡的作用。

Cerebral hemorrhagic rat models were made according to Rosenberg method and the morphosis of neuron in CA 1 region,DNA integrity and the number of withered nerve cells were observed.The results showed that every rat appeared hemiplegic;7 days later,the morphosis of vertebral cell in region CA 1 was almost normal in Naoyi'an group and mostly withered in model one with signifcant differency(P<0.01);DNA cleavage rate in Naoyi'an group was 10%-14% lower than that of model group;the withering of nerve cell in Naoyi'an group was lower than that of model group with signifcant differency(P<0.05).It suggested that Naoyi'an granule could restrain the breaking of DNA and the withering of nerve cell.