野生型金黄色葡萄球菌株norA基因敲除菌株的构建及其耐药性变化

Construction of a NorA Knock-out Strain of Wild-type Staphylococcus Aureus and Preliminary Analysis of Its Antimicrobial Resistance Characteristics

目的探讨norA基因在野生型金黄色葡萄球菌多重耐药中的作用。方法用DNA重组技术构建金黄色葡萄球菌norA基因敲除株并测定其对3种喹诺酮类抗生素的最小抑菌浓度(minimum inhibitory concentration,MIC)值。结果成功构建了野生型金黄色葡萄球菌株的norA基因敲除菌株,且其MIC值均有一定程度下降。结论 norA基因在金黄色葡萄球菌多重耐药中起一定的作用。

Objective To investigate the effect of norA gene on multiple drug-resistance of wild-type staphylococcus aureus.Methods After the construction of a norA knock-out strain of wild-type staphylococcus aureus with DNA recombination,the minimum inhibitory concentrations（MICs） of three quinolones antibiotics aimed at this strain were analysed.Results A norA knock-out strain of staphylococcus aureus was successfully constructed,and the MICs of the three quinolones antibiotics was decreased to some extent.Conclusion NorA gene plays a certain role in multiple drug-resistance of wild-type staphylococcus aureus.