高压氧对糖尿病大鼠血浆内皮素及一氧化氮的影响

Effect of hyperbaric oxygenation on plasma endothelin and nitric oxide in diabetes rats

目的 研究糖尿病大鼠血浆一氧化氮（ＮＯ） 及内皮素（ＥＴ） 的变化规律，探讨高压氧（ＨＢＯ）治疗糖尿病血管并发症的作用机理。方法 观察糖尿病大鼠ＨＢＯ 作用前，１ ，２ 疗程后血浆ＮＯ及ＥＴ 的变化。结果 与正常对照组相比，糖尿病大鼠对照组早期ＮＯ 升高，ＥＴ 降低，晚期ＮＯ降低，ＥＴ升高，差异均有非常显著性，均Ｐ＜ ０．０１。糖尿病大鼠ＨＢＯ作用组２ 疗程后ＮＯ 为（７ ．４５±０．４５）μｍｏｌ／Ｌ，均较作用前（１０ ．３１ ±０．８３）μｍｏｌ／Ｌ及１ 疗程后（９．９３ ±０．８２）μｍｏｌ／Ｌ 明显降低，均Ｐ＜０．０１，ＥＴ（８９．５８±１８．８８）ｎｇ／Ｌ 较作用前（５０ ．６９ ±１２ ．９４）ｎｇ／Ｌ及１ 疗程后（５３ ．１４±１６．１１）ｎｇ／Ｌ均明显升高，均Ｐ＜ ０．０１。结论 糖尿病大鼠旱期出现高ＮＯ低ＥＴ，晚期出现低ＮＯ高ＥＴ，致糖尿病血管并发症发生，ＨＢＯ 通过延缓晚期低ＮＯ 高ＥＴ 病程，平衡血浆ＮＯ及ＥＴ 的含量而达到治疗糖尿病血管并发症的目的。

Objective To study the role of nitric oxide(NO) and endothelin(ET) in diabetes rats,and explore the effect and mechanism of hyperbaric oxygenation(HBO) for vascular complications of diabetes.Methods Before HBO and after 1 or 2 courses of HBO treatment,the NO and ET level of diabetes rats was measured.Results Compared with the normal group,the NO level raised and ET level reduced in early diabetes rats,contrarily,the NO reduced and ET raised in late diabetes rats(P<0.01).At pre-HBO,the NO and ET level was (10.31±0.83)μmol/L and (50.69±22.94)ng/L respectively;after 2 courses of treatment,the NO and ET level was (7.45±0.45)μmol/L and (89.58±18.88)ng/L separately;after 1 course of HBO,the NO was (9.93±0.82)μmol/L and ET was (53.14±16.11)ng/L.Among them,there was a significant difference(P<0.01).Conclusion It shows that the diabetes complications are related to the abnormal NO and ET level.The HBO therapy may regulate the balance of NO or ET to obtain the recovery of diabetes complications.The more the treatment is,the effectiveness will be better.