远端缺血预处理在小体积肝移植物缺血-再灌注损伤的保护作用

Protective effect of remote ischemia preconditioning on ischemia-reperfusion injury in a small-for-size liver transplantation model

目的研究远端缺血预处理(RIPC)在小体积肝移植物缺血-再灌注(I-R)损伤中的保护作用。方法将10只大鼠随机分成两组,每组5只,原位肝移植组(A组)和RIPC后肝移植组(B组)。建立30%大鼠肝移植模型,检测术后2、6、12、24 h血清中ALT的水平,RT-PCR、Westernblot分别检测肝脏组织中血红素加氧酶1(HO-1)mRNA和蛋白表达。结果与A组相比,术后B组血清ALT水平下调,HO-1 mRNA及蛋白表达均明显增加(P<0.05)。结论 RIPC具有保护小体积移植物I-R损伤作用,这可能与HO-1在术后肝脏组织中的过表达有关。

Objective To investigate the protective effect of remote ischemia preconditioning（RIPC） on ischemia-reperfusion （I-R） injury in a small-for-size liver transplantation model.MethodsTen rats were randomly and equally divided into two groups of A（orthotopic liver transplantation） and B（liver transplantation after RIPC）.The 30% liver transplantation model in rats was used,and the level of alanine aminotransferase（ALT） was measured at 2,6,12,24h after operation.The expressions of hemeoxygenase-1（HO-1） mRNA and protein were detected by RT-PCR and Western blot,respectively.Results Compared with group A,the level of ALT was decreased,whereas the expressions of HO-1 mRNA and protein were significantly increased after transplantation in group B（P0.05）.Conclusion RIPC can protect I-R injury in small-for-size liver transplantation,which may be related with the over-expression of HO-1 in the liver after operation.