伴放线放线杆菌对2型糖尿病患者多形核白细胞分泌白细胞介素-6和凋亡的影响

Effect of Actinobacillus actinomycetem on the secretion of interleukin-6 and apoptosis rate of polymorphonu-clear leukocyte in type 2 diabetes patients

目的研究2型糖尿病患者多形核白细胞(PMN)分泌功能和凋亡情况以及伴放线放线杆菌(A.actinomycetem)对其的影响。方法 Percoll不连续梯度离心法分离非糖尿病者和2型糖尿病患者外周血中的PMN,实验组的PMN加入A.actinomycetem的超声滤液刺激,对照组为PMN的PBS悬浮液。培养20、40、60 min后定量酶联免疫吸附试验(ELISA)检测其白细胞介素-6(IL-6)的分泌水平;另外在培养6、12 h后使用流式细胞分析仪检测各组PMN的凋亡率。结果糖尿病患者的PMN无论是否受到A.actinomycetem超声滤液刺激,在实验各个时段的IL-6分泌水平均高于非糖尿病者,且细胞凋亡率明显降低(P<0.001)。结论 2型糖尿病患者的PMN具有过度炎症反应特征,在受到牙周致病菌刺激后,凋亡推迟,分泌炎症介质,从而造成严重的牙周组织破坏。

Objective To study the effect of Actinobacillus actinomycetem（A.actinomycetem） on the secretion and apoptosis rate of polymorphonuclear leukocytes（PMN） in type 2 diabetes patients.Methods Peripheral PMN from healths and type 2 diabetes patients were isolated by Percoll gradient centrifugation.The PMN were stimulated with fil-trate of ultrasonic pulverization from A.actinomycetem as the experiment group.As the control group,PMN suspension was incubated with PBS.The release of interleukin-6（IL-6） was measured at 20,40,60 min by enzymelinked immune sorbent assay（ELISA） technique.The apoptosis rate of PMN was tested at 6 and 12 hours by flow cytometry.Results Incubated with filtrate of ultrasonic pulverization from A.actinomycetem,the PMN of type 2 diabetes patients released significantly higher levels of IL-6 compared with the healthy subjects（P0.001）.The apoptosis rate of PMN from the healthy subjects was higher than that from type 2 diabetes patients（P0.001）.Regardless of body condition,interaction with filtrate of ultrasonic pulverization from A.actinomycetem could induce the seretion of IL-6 and reduce the apoptosis rate.Conclusion The PMN of type 2 diabetes patients may possess hyper-reactive inflammatory response trait.