期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

PGC-1β和SREBP-1c在猪脂肪细胞分化过程中的相互作用 被引量：5

Interaction of PGC-1β and SREBP-1c during Porcine Adipocyte Differentiation
下载PDF
导出
摘要 为研究过氧化物酶体增殖物激活受体γ辅激活因子1β(PGC-1β)与SREBP-1c在猪前体脂肪细胞分化过程中的表达规律及其相互作用,分析二者功能上的联系,采用Western印迹及细胞免疫荧光技术检测PGC-1β与SREBP-1c在猪脂肪细胞分化过程中的表达,shRNA干扰和免疫共沉淀技术分别探讨了PGC-1β对SREBP-1c的调节作用及2种蛋白质在体内的结合活性.结果显示,PGC-1β与SREBP-1c蛋白的表达均随猪脂肪细胞分化逐渐增加,且在分化细胞的核和胞浆中均有分布.干扰PGC-1β显著下调了SREBP-1c和脂肪细胞分化标记基因C/EBPα的表达(P<0.05),同时降低了细胞内甘油三酯的积累.免疫共沉淀证明,PGC-1β与SREBP-1c蛋白在猪脂肪细胞分化过程中存在结合作用.以上结果表明,PGC-1β能够促进猪脂肪细胞分化并对SREBP-1c有调节和结合作用,推测二者的结合可能与其对脂肪细胞的分化调节机制相关,将对PGC-1β调控脂肪细胞分化的功能和机理研究提供新途径. To study the interaction and function of peroxisome proliferator-activated receptor-γ coactivator-1β（PGC-1β） and sterol regulatory element binding protein 1c（SREBP-1c） during porcine preadipocyte differentiation,the expression of PGC-1β and SREBP-1c were detected by Western blotting and immunofluorescence.The regulation of PGC-1β on the SREBP-1c and the binding activity between the two proteins in vivo were analyzed using shRNA technology and co-immunoprecipitation.The results showed that PGC-1β and SREBP-1c increased gradually during porcine adipocyte differentiation,and the two proteins located both in the nucleus and cytoplasm.PGC-1β interference significantly decreased（P0.05） the expression of SREBP-1c and C/EBPα,an adipocyte differentiation marker gene,and reduced the accumulation of triglyceride.Further analysis showed that PGC-1β and SREBP-1c could be co-immunoprecipitated.We conclude that PGC-1β interacts with SREBP-1c,and may regulate adipocyte differentiation.
作者 高晓娟 吉红 常志光 刘月光 杨秋梅 杨公社
机构地区 西北农林科技大学动物科技学院动物脂肪沉积与肌肉发育实验室
出处 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2011年第6期533-539,共7页 Chinese Journal of Biochemistry and Molecular Biology
基金 转基因生物新品种培育重大专项(No.2008ZX08006-005 No.2009ZX08009-157B) 西北农林科技大学"创新团队建设计划"资助~~
关键词 过氧化物酶体增殖物激活受体γ辅激活因子1β(PGC-1β) 固醇调节元件结合蛋白1C 脂肪细胞 分化 相互作用 peroxisome proliferator-activated receptor-γ coactivator-1β（PGC-1β） sterol regulatory element binding protein 1c（SREBP-1c） adipocyte differentiation interaction
分类号 Q78 [生物学—分子生物学] Q245 [生物学—细胞生物学]
  • 相关文献

参考文献20

  • 1Lin J, Puigserver P, Donovan J,et al. Peroxisome proliferator-activated receptor gamma eoactivator 1 beta ( PGC-1beta ), a novel PGC-1-related transcription coactivator associated with host cell factor[ J ]. J Biol Chem, 2002,277 ( 3 ) : 1645-1648.
  • 2吉红,卢荣华,常志光,杨公社.转录辅助活化因子PGC-1家族的生物学特性及功能[J].中国生物化学与分子生物学报,2010,26(7):596-603. 被引量：12
  • 3Lin J, Handscbin C, Spiegeman B M. Metabolic control through the PGC-1 family of transcription coactivators [ J]. Cell Metab, 2005,1(6) :361-370.
  • 4Cooper MP, Uldry M, Kajimura S, et al. Modulation of PGC-1 coaetivator pathways in brown fat differentiation through LRP130 [J]. J Biol Chem, 2008,283(46) :31960-31967.
  • 5Kamei Y,Ohizumi H, Fujitani Y, et al. PPARgamma coactivatorl beta/ERR ligand 1 is an ERR protein ligand,whose expression induces a high-energy expenditure and antagonizes obesity [ J ]. Proc Nat1 Acad Sci U S A,2003,100(21 ) :12378-12383.
  • 6李影.SREBPs调控体脂平衡分子机制研究进展[J].安徽农业科学,2007,35(20):6041-6043. 被引量：8
  • 7Gondret F, Ferre P, Dugail I. ADD-1/SREBP-1 is a major determinant of tissue differential lipogenic capacity in mammalian and avian species [ J ]. J Lipid Res, 2001,42 ( 1 ) : 106-1 ! 3.
  • 8Becard D, Hainauh I, Azzout-Marniche D, et al. Adenovirus- mediated overexpression of sterol regulatory element binding protein-1c mimics insulin effects on hepatic gene expression and glucose homeostasis in diabetic mice [ ] ]. Diabetes, 2001,50 ( 11 ) :2425-2430.
  • 9Steffensen K R, Gastafsson J A. Putative metabolic effects of the liver X receptor(LXR) [ J ]. Diabetes,2004,53 ( Suppl 1 ) : S36-d2.
  • 10Payne V A, Au W S, Lowe C E, et al. C/EBP transcription factors regulate SREBPlc gene expression during adipogenesis [ J]. Biochem J,2009,425 ( 1 ) :215-223.

二级参考文献89

  • 1Yoon J C, Puigserver P, Chen G, et al.Control of hepatic gluconeogenesis through the transcriptional coactivator PGC-1[J].Nature.2001,413(6852):131-138.
  • 2Hara K,Tobe K,Okada T, et al.A genetic variation in the PGC-1 gene could confer insulin resistance and susceptibility to type Ⅱdiabetes [J].Diabetologia.2002, 45(5):740-743.
  • 3Goyenechea E, Crujeiras A B, Abete I, et al.Enhanced short-term improvement of insulin response to a low-caloric diet in obese carriers the Gly482Ser variant of the PGC-1a gene [J].Diabetes Res Clin Pract.2008, 82(2):190-196.
  • 4Kamei Y, Ohizumi H, Fujitani Y, et al.PPAR{gamma} coactivator1{beta} /ERR ligand 1 is an ERR protein ligand, whose expression induces a high-energy expenditure and antagonizes obesity[J].Proc Natl Acad Sci U S A.2003, 100(21):12378-12383.
  • 5Nobuhiro I, Kazuhiro I, Kuniko H I, et al.Estrogen-related receptor alpha modulates the expression of adipogenesis-related genes during adipocyte differentiation[J].Biochem Biophys Res Commun.2007, 358(3):813-818.
  • 6Cooper M P, Uldry M, Kajimura S, et al.Modulation of PGC-1 coactivator pathways in brown fat differentiation through LRP130[J].J Biol Chem.2008, 283(14):31960-31967.
  • 7Andersen G, Wegner L, Yanagisawa K, et al.Evidence of an association between genetic variation of the coactivator PGC-1β and obesity[J].J Med Genet.2005, 42(5):402-407.
  • 8Lu R H, Ji H, Chang Z G, et al.Mitochondrial development and the influence of its dysfunction during rat adipocyte differentiation [J].Mol Biol Rep,2009 Aug 20,(Epub ahead of print).
  • 9Finck BN, Kelly D P.PGC-1 coactivators:inducible regulators of energy metabolism in health and disease[J].J Clin Invest.2006, 116(3):615-622.
  • 10St-Pierre J, Lin J, Krauss S, et al.Bioenergetic analysis-1 of peroxisome proliferator-activated receptor gamma coactivators-l alpha and -1 beta (PGC-1alpha and PGC-1beta) in muscle cells[J].J Biol Chem.2003, 278(29):26597-26603.

共引文献60

同被引文献98

引证文献5

二级引证文献14

中国生物化学与分子生物学报
2011年 第6期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部