淋球菌流行株对抗生素的耐药性研究

Study on the Resistance of Neisser in Gonorrhoeae Epidemic Strains to Antibiotics

目的：了解淋球菌流行株对抗生素的耐药性。方法：对１９９８ ～１９９９ 年间广东省湛江地区分离出的９８ 株淋球菌流行株，在系统鉴定的基础上，进行了抗生素敏感性的测定，分别用纸片扩散法和琼脂稀释法测定青霉素、四环素、壮观霉素、头孢三嗪和环丙沙星的抑菌圈直径（ ｍ ｍ ） 和最小抑菌浓度（ ＭＩＣ） ，根据ＮＣＣＬＳ 标准判定药物敏感性。结果：纸片扩散法测定淋球菌对这５ 种抗生素的耐药百分率分别为３２－６５ ％ 、６９－３９ ％ 、８－１６ ％ 、１３－２７ ％ 和８２－６５ ％ ，而琼脂稀释法测得相应的耐药 百分 率为 ２３－９１ ％ 、４９－４６ ％ 、１１－１１ ％ 、１６－４８ ％ 和５９－３４ ％ 。结论：湛江地区目前流行的淋球菌菌株耐药状况十分严重，尤以环丙沙星为甚，临床常用的头孢三嗪。

OBJECTIVE To measure the antibiotic susceptibility of neisser ia gonorrhoeae epidemic strains in Zhanjiang(Guangdong).METHODS 98 gonococcal isolated obtained from 1998 through 1999 in Zhanjiang were tested for theirantibiotic susceptibilities based on the systemic identification.The inhibitory zone diameters(mm) and the minimum inhibitoryconcenrations(MICs)of penicillin tetracycline,spectinomycin,ceftr iaxone and ciprofloxacin were determined by using disk diffusion and agar dilution methods,respectively.Then difined the susceptibilities of all these isolates using the MCCLS criteria.RESULTS The percentages of gonococci resitant strains to penicillin,tetracycline,spectinomycin,ceftriaxion and ciprofloxacin were 32 65%、69 39%、8 16%、13 27% and 82 65% by using disk diffusion metod.At the same tiome,the percentages of resistant strains by using agar dilutiion method were 23 91%、49 46%、11 11%、16 48% and 59 34% respectively.CONCLUSION The resistant strains of contemporary gonococci in Zhanjiang were serious.especially the ciprofloxacin resistance.By the way.contimued active survillance is needed to detect and control the spread of ceftr iaxone and spectinomycin resistant Neisseria gonorrhoeae.