骨桥蛋白mRNA在大肠癌患者外周血中的表达及其临床意义

Expression of osteopontin mRNA in peripheral blood from patients with colorectal carcinoma and its clinical significance

目的检测大肠癌外周血中的骨桥蛋白(OPN)mRNA的表达,探讨其与临床病理分期及淋巴转移的关系。方法应用逆转录聚合酶链反应技术检测52例大肠癌患者(大肠癌组)外周血单个核细胞中OPN mRNA的表达,并以33例大肠腺瘤患者(大肠腺瘤组)及20例健康人(健康对照组)外周血作为对照。结果 OPN mRNA在大肠癌组外周血单个核细胞中表达的阳性率为65.4%,大肠腺瘤组OPN mRNA阳性率为33.3%,健康对照组外周血中均无靶OPN mRNA表达。大肠癌组与大肠腺瘤组OPN mRNA阳性表达率均高于健康对照组(P均<0.01),大肠癌组OPN mRNA阳性表达率高于大肠腺瘤组(P<0.05)。随着大肠癌Dukes分期的升高,其外周血单个核细胞中OPN mRNA表达阳性率逐渐增高(P<0.05)。有淋巴结转移的表达阳性率高于无淋巴结转移及对照组(P均<0.01);无淋巴结转移患者与大肠腺瘤组之间差异无统计学意义。结论外周血单个核细胞中OPN mRNA阳性表达对大肠癌的淋巴转移及预后判断具有一定价值。

Objective To detect the expression of osteopontin （OPN） mRNA in peripheral blood from patients with colorectal carcinoma and explore its association with clinico-pathological stage and lymphatic metastasis.Methods Peripheral blood mononuclear cells were isolated from 52 patients with colorectal carcinoma （colorectal carcinoma group）,33 patients with colorectal adenoma （colorectal adenoma group） and 20 healthy volunteers （healthy control group）.The expression of OPN mRNA in peripheral blood mononuclear cells was detected with reverse transcriptase polymerase chain reaction （RTPCR）.Results The positive expression rate of OPN mRNA in peripheral blood mononuclear cells was 65.4% （34 /52） in colorectal carcinoma group,and 33% （11 /33） in colorectal colorectal adenoma group,while none was detected in healthy control group.The positive expression rates of OPN mRNA in colorectal carcinoma group and adenoma group were all higher than those in healthy control group （all P 〈 0.01）,and positive expression rate of OPN mRNA in colorectal carcinoma group was higher than that in colorectal adenoma group （P 〈 0.05）.The positive expression rates of OPN mRNA in colorectal carcinoma group gradually increased with the progress of Dukes stages （P 〈 0.05）.The OPN mRNA positive expression rate of patients with lymphatic metastasis was higher than those of patients without lymphatic metastasis in colorectal carcinoma group and in healthy control group （all P 〈 0.01）,and there was no statistical significance in positive expression rate of OPN mRNA between patients without lymphatic metastasis in colorectal carcinoma group and colorectal adenoma group.Conclusions OPN mRNA positive expression in mononuclear cells isolated from peripheral blood of colorectal carcinoma patients has a certain clinical value for judging lymphatic metastasis and prognosis of colorectal carcinoma due to it might be a marker of micrometastasis in colorectal carcinoma.