重楼中薯蓣皂甙元的反相高效液相色谱测定

Determination of Diosgenin in Rhizoma Paridis by High Performance Liquid Chromatography

采用高效液相色谱法测定了重楼中薯蓣皂甙元的质量分数。样品先经甲醇提取，再经酸水解，将重楼甾体皂甙转变成薯蓣皂甙元，以 Ｓｙｍ ｍ ｅｔｒｙ Ｃ８ 柱为色谱柱，以 Ｖ（乙腈）∶ Ｖ （水）＝ ７５∶２５ 溶液为流动相，检测波长２０３ ｎｍ ，重复性较好。

A method for the separation and determination of diosgenin in Rhizoma Paridis by reversed phase high performance liquid chromatography was developed. The Rhizoma Paridis powder samples were extracted with methanol in a Soxhlet extractor. After extraction the solvent was evaporated and the extract was hydrolysed with 2 mol/L hydrochloric acid for 2 h in a boiling water bath. Then the diosgenin was extracted with petroleum ether(b.p.60 90 ℃). The operating conditions were Symmetry C 8 column(5 μm,3.9 mm×150 mm)at 30 ℃, mobile phase of V(acetonitrile)∶V(water)=75∶25 and UV detector at 203 nm. The linearity of the calibration curve was good in the range of 2.1 10.5 μg for diosgenin(r=0.999 4). The average recovery and RSD of diosgenin were 99.1% and 1.7% (n=5) respectively. The method is accurate and reproducible and has been applied to the analysis of Rhizoma Paridis from different sources.