期刊文献+

巨噬细胞源性泡沫细胞适配子PM1的特异性 被引量:5

Research on the Specificity of PM1 Aptamer About THP-1 Macrophage Derived Foam Cells
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摘要 目的研究特异性寡核苷酸适配子PM1与巨噬细胞源性泡沫细胞和动脉粥样硬化病变结合的特异性,为动脉粥样硬化的体内靶向治疗提供实验依据。方法对适配子PM1进行FITC标记,荧光显微镜分别观察适配子与血管平滑肌细胞、巨噬细胞、内皮细胞、巨噬细胞源性泡沫细胞结合情况。采用高脂方法建立兔动脉粥样硬化模型,利用PCR方法和荧光显微镜观察适配子与动脉粥样硬化病变结合情况。结果适配子PM1与巨噬细胞源性泡沫细胞和动脉粥样硬化病变组织高度特异性结合,但不结合血管平滑肌细胞、巨噬细胞、内皮细胞和正常动脉组织。结论筛选出的适配子PM1能特异性结合巨噬细胞源性泡沫细胞和动脉粥样硬化病变。 Aim To observe the binding specificity of aptamers and THP-1 macrophage derived foam cells and atheroselerotic lesions. Methods The FITC-labeled PM1, binding to THP-1 macrophages, vascular smooth cells, endothelial cells and THP-1 macrophage derived foam cells were observed by fluorescence microscopy respectivly. Model of atherosclerosis was formed by high-fat feeding rabbit methods. The binding of aptamers and atherosclerotic lesions were detected by fluorescence microscopy and PCR. Results PM1 didn' t bind to THP-I macrophages, vascular smooth ceils and endothelial cells but bind to THP-1 macrophage derived foam cells and atheroselerotic lesions. Conclusions we have obtained PM1 specifically binding to THP-1 macrophage derived foam cells and atheroselerotic lesions.
出处 《中国动脉硬化杂志》 CAS CSCD 北大核心 2011年第5期405-408,共4页 Chinese Journal of Arteriosclerosis
关键词 适配子 巨噬细胞源性泡沫细胞 动脉粥样硬化病变 Aptamer Macrophage Derived Foam Cell Atherosclerotic Lesion
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参考文献14

  • 1Li AC, Glass CK. The macrophage foam cell as a target for herapeteutic Intervention[J]. Nat Med, 2002, 8 ( 11 ) : 1 235 -242.
  • 2Zimmermann B, Gesell T, Chen D, et al. Monitoring ge- nomic sequences during SELEX using high-throughput se- quencing: neutral SELEX[J]. PLoS One, 2010, 5 (2): e9169.
  • 3Sefah K, Meng L, Lopez-Colon D, et al. DNA aptamers as molecular probes for colorectal cancer study [J]. PLoS One, 2010, 5 (12) : e14269.
  • 4Hicke BJ, Stephens AW. Escort aptamers: a delivery, service for diagnosis and therapy [ J ]. J Clin Invest, 2000,106 (8) : 923-928.
  • 5Clark SL, Remcho VT. Aptamers as analytical reagents [J]. Electrophoresis, 2002, 23 (9) : 1 335-340.
  • 6Joshi P, Prasad VR. Potent inhibition of hmnan immunode- ficiency virus type 1 replication by template analog reverse transcriptase inhibitors derived by SELEX (systematic evo- lution of ligands by exponential enrichment) [ J]. J Viral, 2002, 76 (13) : 6 545-557.
  • 7Sung Ho Jeon, Basak Kayhan, Tamar Ben-Yedidia, et al. A DNA aptamer prevents influenza infection by blocking the receptor binding region of the viral hemagglutinin[ J]. J Bi- ol Chem, 2004, 276 (49) : 48 410-419.
  • 8Charlton J, Sennello J, Smith D. In vivo imaging of inflam- mation using an aptamer inhibitor of human neutrophil elas- tase[Jl. J Chem Biol, 1997, 4: 809-816.
  • 9张玮,席艳,孙慧君,汤新强.脂蛋白A与血栓及动脉粥样硬化的关系[J].中国现代医学杂志,2007,17(20):2500-2502. 被引量:5
  • 10严鹏科,廖端芳,杨永宗.Caveolin-1表达对血管平滑肌细胞胆固醇逆转运的调节作用[J].中国动脉硬化杂志,2002,10(5):379-383. 被引量:34

二级参考文献19

  • 1翟雪松,朱红枫,邹艳,胡晓.小儿肾病综合征低密度脂蛋白(LDL)与脂蛋白(a)[LP(a)]的变化及其临床意义[J].海南医学,2006,17(1):50-51. 被引量:2
  • 2张清德,张以卿,王先立.通心络胶囊对冠心病患者Lp(a)等血脂的影响[J].菏泽医学专科学校学报,2005,17(4):15-17. 被引量:2
  • 3董军,陈文祥,李健斋.高效液相色谱测定微量胆固醇氧化产物[J].生物化学与生物物理进展,1996,23(2):179-182. 被引量:9
  • 4BERG K.A new serum type system in man:the LPa system[J].Axta Pathol Microbiol Scand 1963;59:362-382.
  • 5ROSS R.The pathogenasis of atherosclerosis:a perspective for the 1990's[J].Nature 1993;362:801-9.
  • 6FLESS GM,ZUMALLEN ME,SCANU AM.Physicochemical properties of apolipoproteinA and LipoproteinA derived form the dissociation of human plasma lipopmteinA[J].J Biol Chem 1986; 261:8712-18.
  • 7BOFFA MB,MARCOVINA SM,KOSCHINSKY ML.Lipoprotein (a) as a risk factor for atherosclerosis and thrombosis:mechanistic insights from animal models[J].Clin Biochem,2004,37(5):333.
  • 8DURRING TP.LinoproteinA Bailliere's Clin Endocrinol Metab 1995;9:773-95.
  • 9SCANO AM,NAKAJIMA K,EDELSTEIN C.ApoproteinA and biology[J].Front Biosci,2002,6:D546-554.
  • 10HOFF HF,O'NEIL J,YASHIRO A.Partial characterization of lipoproteins containing aopA in human atherosclerotic lesinons[J].J Lipid Res 1993;34:789-98.

共引文献37

同被引文献67

  • 1徐正云,任国珍,马爱群.大鼠胸主动脉平滑肌细胞的培养与鉴定[J].岭南心血管病杂志,2005,11(3):202-204. 被引量:11
  • 2汪江波,张慧,严鹏科.指数富集的配基系统进化技术及其在血管研究中的应用[J].中国动脉硬化杂志,2006,14(3):267-270. 被引量:1
  • 3祝军,张景海.生物素-亲合素ELISA法对ANEPⅢ寡核苷酸适配子亲和力的定量测定[J].细胞与分子免疫学杂志,2007,23(6):580-581. 被引量:4
  • 4Andrew C Li, Christopher K.Glass.The macrophage foam cell as a target fortherapeutic intervention[J].Nat Med,2002,8 ( 11 ): 1235-1242.
  • 5Tuerk C, Gold L.Systeraatic evolution of ligands by exponential enrichraent:RNAligands to bacteriophage T4 DNA polymerase[J].Science, 1990,249 ( 4968 ):505-510.
  • 6Sefah K, Shangguan D, Xiong X, et al.Deveiopment of DNA aptamers usingCell-SELEX[J].Nat Protoc ,2010,5 : 1169-1185.
  • 7Choudhury RP, Lee JM, Greaves DR.Mechanisms of disease:macrophage-derived foam cells emerging as therapeutic targets in atherosclerosis[J].NatureClinical Practice.Cardiovascular Medicine, 2005,2 ( 6 ): 309-315.
  • 8Kim D, Jeong YY, Jon S.A drug-loaded aptamer-gold nanoparticlebioconjugate for combined CT imaging and therapy, of proctate cancer(J].ACSNano,2010,4 (7): 3689-3696.
  • 9H wang do W, Ko HY, Lee JH, et al.A nucleolin-targeted multimodalnanoparticle imaging probe for tracking cancer cells using an aptamer[J].J Nuclmedm,2010,51 (1 ): 98-105.
  • 10Chan MY, Rusconi CP, Alexander JH, et al.A randomized, repeat-dose,pharmacodynamic and safety study of an antidote-controlled factor IXainhibitor[J].J Thromb Haemost,2008,6 ( 5 ): 789-796.

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