组织工程细胞片构建方法

CONSTRUCTION METHODS OF TISSUE ENGINEERED CELL SHEET

目的综述组织工程细胞片构建方法的研究进展并展望前景。方法广泛查阅近年来有关构建组织工程细胞片的研究与应用的文献,选择常见方法分别进行阐述。结果通过组织工程细胞片方法构建的组织,可以打破传统组织工程方法对组织工程发展的限制。目前构建组织工程细胞片的方法主要包括温敏培养皿、胶原凝胶、磁力组织工程、表面粗糙的颗粒单分子层及聚合电解质。结论 组织工程细胞片构建方法可行,具有广泛的应用前景。

Objective To summarize the recent progress of construction methods of engineered cell sheet and to forecast the possible prospect. Methods The recent original articles about investigation and application of engineered cell sheet were reviewed. Several common methods were selected and expounded. Results The construction methods of engineered cell sheet mainly include temperature-responsive culture dish, salmon atelocollagen, magnetic force, surface roughness, and polyelectrolytes, which may overcome the limits of traditional tissue engineering methods. Conclusion The construction methods of engineered cell sheet are feasible and have a bright future in the clinical application.