摘要
本文建立了食品中胭脂树橙含量的超高效液相色谱紫外检测方法。样品采用酸化乙腈溶液提取,提取液经乙腈饱和正己烷萃取净化后注入超高效液相色谱仪,由BEH C18柱(1.7μm,2.1×50mm)快速分离后进入紫外检测器检测。本方法胭脂树橙检测限为0.1mg/kg。5种食品样品回收率为83.8%～96.1%,相对标准偏差(RSD)为0.6%～6.1%(n=6),在0.1μg/mL～2μg/mL浓度范围内呈良好的线性关系,线性回归系数r=0.9999,结果令人满意。
A method for determination of Bixin in food using ultra high performance liquid chromatography had been developed.The sample was extracted by acidified acetonitrile,cleaned up with hexane and determined by UPLC-UV.The separation column was used with Waters BEH C18（1.7μm,2.1×50mm）.The detection limit was 0.1mg/kg.The recovery ranged from 83.8% to 96.1% with five food samples,the relative standard deviation（RSD） was 0.6%~6.1%（n=6）.The good linearity was achieved in 0.1μg/mL ~2μg/mL with r=0.9999,the result was satisfactory.
出处
《福建分析测试》
CAS
2011年第3期36-39,共4页
Fujian Analysis & Testing
基金
国家质量监督检验检疫总局自筹经费项目(T2009-FJQTS-0064)
