血水草生物碱的HPLC分离

HPLC Separation of Alkaloids in Eomecon Chionantha Hance

建立了内衬聚亚胺型Ｃ１８ 反相高效液相柱分离血水草生物碱ＨＰＬＣ 新方法。 考察了梯度洗脱分离时，起始缓冲液组成及ｐＨ 值对分离的影响。 当用０ ．０２８ ｍｏｌ／Ｌ Ｋ２ ＨＰＯ４（ｐＨ ８ ．５） 为起始洗脱液， 线性梯度洗脱至１００ ％ 甲醇时， 血水草提取液中主要生物碱以及含量较少的生物碱可得到理想分离。 用峰面积归一化法考察不同地点， 不同季节血水草各生物碱含量的变化规律， 为血水草的采收提供了科学依据。

A new reverse _ phase HPLC method for the separation of quaternary alkaloids in Eomecon chionantha Hance is described. A C18 column with silica _ based packing pre _ shielded with polyimide is employed. The effects of initial buffer composition and pH on the gradient elution have been investigated. The major alkaloids can be separated from minor constituents under a linear gradient elution mode. The initial eluate is 0.028 mol/L K2HPO4 at pH 8.5, whereas the final eluate is 100% methanol. It is found that the contents of alkaloids in the herb from different places are different, and vary with growing seasons too.