花生中黄曲霉毒素B_1、B_2、G_1、G_2的多功能净化柱-高效薄层色谱分析

Determination of Aflatoxins B_1、 B_2、 G_1、 G_2 in Peanuts by High Performance Thin Layer Chromatography with Multifunction Clean _ up Column

建立了一种测定食品中黄曲霉毒素（ ＡＦＴ） Ｂ１ 、Ｂ２ 、Ｇ１ 、Ｇ２ 的多功能净化柱（ ＭＦＣ） 净化， 单相展开的薄层色谱法。 以乙腈－ 水（ 体积比９ ∶１） 提取样品中ＡＦＴ， 经ＭＦＣ 净化浓缩后， 采用ＨＳＧ６０ 薄层板， 以丙酮－ 氯仿（ 体积比９ ∶１） 展开， 薄层扫描仪荧光检测扫描定量。该法用于花生样品的检测，４ 种毒素的分离良好； 线性范围：０ ．０５ ～１ ．０ ｎｇ， 相关系数≥０ ．９９９ １； 检出限均达到０ ．５ ×１０ － ９ ； 相对标准偏差为４ ．６７ ％ ～１０ ．２１ ％ ； 样品加标０．５ ×１０ － ９ ～１０ ×１０ － ９ ， 平均回收率为８６ ．５ ％ ～９９ ．０％ 。

A method for the determination of aflatoxin B1、 B2、 G1 and G2 by high performance thin layer chromatography with multifunction cleanup column(MFC) was developed. Samples are extracted with aqueous actonitrile solution(φ=90%)and clean up with the MFC. Aflatoxins pass through directly the column and the coextracted materials are retained. The purified solution is concentrated and spotted on the high performance silica gel 60 plate. Developing with acetone-chloroform(volume ratio 9∶1) in one direction, the aflatoxins are quantified with TLC fluorescence scanner. The method has been applied to the determination of peanut sample with four toxins separating well. The linear range of the method is 0.05～1.0 ng, the correlation coefficient ≥ 0.999 1, the detection limit 0.5×10-9 for each toxin, the relative standard deviation 4.67%～10.21% and the average recovery of AFT spiked at 0.5×10-9 ～ 10.0×10-9 levels is in the range of 86.5%～99.0%.