3-羟基-4-甲氧基肉桂酸——基体辅助激光解吸电离质谱法测定DNA的有效基体

A Kind of Effective Matrix 3 _ Hydroxy _ 4 _ methoxycinnamic Acid in Measurement of DNA by Matrix Assisted Laser Desorption Ionization Mass Spectrometry

报道用基体辅助激光解吸电离质谱法（ ＭＡＬＤＩ－ ＭＳ） 测定ＤＮＡ 分子的一种有效基体３－ 羟基－ ４ － 甲氧基肉桂酸。 实验发现该基体对ＤＮＡ 分子解吸和电离效率高， 对ＤＮＡｄ（ Ｔ） １０ 分子离子峰的分辨率为５ ５５１ ．４ ， 信噪比为１１ ．８ 。３ － 羟基－ ４ － 甲氧基肉桂酸是ＭＡＬＤＩ－ ＭＳ 法测定ＤＮＡ 分子的一个可供选择使用有效基体。

A kind of effective matrix, 3 _ hydroxy _ 4 _ methoxycinnamic acid, in measurement of DNA by matrix assisted laser desorption ionization mass spectrometry(MALDI-MA) is reported. The results show that 3 _ hydroxy _ 4 _ methoxycinnamic acid has high efficiency in desorbing and ionizing DNA molecules. For the molecular ion peak +of d(T)10, the resolution is 5 551.4,and the signal _ to _ noise is 11.8. 3 _ Hydroxy _ 4 _ methoxycinnamic acid is a good alternative matrix in the analysis of DNA by MALDI-MS.