摘要
目的研究多发性骨髓瘤(MM)患者外周血CD4+T淋巴细胞亚群特点。方法用流式细胞术检测30例MM初诊患者、20例治疗后患者及10名健康志愿者(对照组)外周血CD4+T淋巴细胞亚群,比较分析3组之间调节性T细胞(Treg)、辅助性T细胞(Th)1、Th17比例及Treg/Th17、Th1/Th17比值。结果初诊MM患者外周血Th17比例明显高于对照组,Treg、Th1比例及Treg/Th17、Th1/Th17比值明显低于对照组。治疗后患者Treg、Th1比例及Treg/Th17、Th1/Th17比值较治疗前明显提高,Th1及CD4+CD25+细胞比例基本恢复至对照组水平,CD4+Foxp3+细胞比例及Treg/Th17、Th1/Th17比值仍明显低于对照组。结论 MM患者外周血Th17细胞在初始T细胞的分化过程中取得了极化优势。治疗后Th17细胞极化优势现象明显改善,Th17细胞可能参与了MM的疾病进程。
Objective To study the characteristics of CD4＋T lymphocyte subsets in peripheral blood from the patients with multiple myeloma（MM）.Methods The CD4＋ T lymphocytes subsets from the 30 cases in diagnosis the 20 post-treatment patients with MM and 10 healthy volunteers（control groups） were measured by flow cytometry,The percentage of Treg,Th1 and Th17 cells and the ratios of Treg/Th17,Th1/Th17 were analyzed among the three groups.Results The percentage of Th17 from the cases in diagnosis was clearly higher than that from healthy controls group.The percentage of Th1 and Treg,the ratios of Th1/Th17 and Treg/ Th17 from the cases in diagnosis were markedly lower than those from healthy controls group,and those index from the post-treatment cases were obviously higher than those from the cases in diagnosis.The percentage of Th1 and CD4＋ CD25＋ cells from the post-treatment cases recovered nearly to the levels of healthy controls,The percentage of CD4＋Foxp3＋ cells and the ratios of Th1/Th17 and Treg/Th17 from the post-treatment cases were still lower than those from healthy controls group.Conclusion Th17 cells in peripheral blood from patients with multiple myeloma make a polarizing advantage in the process of naive T cells differentiated into Th1,Treg and Th17.The polarizing advantage of Th17 cells is dramatically improved by treatment,and it is possible that Th17 cells are participated in the development of MM.
