大鼠多重脑震荡后脑内TPH表达变化

Changes of TPH expression after multiple cerebral concussion in rat brain

目的：探讨多重脑震荡（multiple cerebral concussion,MCC）后脑内5-HT能神经元内色氨酸羟化酶（tryptophan hydroxylase,TPH）含量的变化。方法：42只雄性大鼠随即分为7组,每组6只,一组为对照组,其余的大鼠用金属单摆式机械打击装置复制MCC大鼠模型,伤后分1~24 d不同时段的6个损伤组,用免疫组化技术和图像分析方法观察基底前脑及脑干5-HT能神经元内TPH含量的变化。结果：多重脑震荡后2~8 d上述脑区TPH免疫阳性表达升高（P〈0.05）,伤后24 d趋于正常（P〉0.05）。结论：多重脑震荡后5-HT神经元TPH表达增强,可能与脑损伤后神经元的应激反应有关。

Objective： To explore the content change of tryptophan hydroxylase（TPH） in cerebral serotoninergic neurons after multiple cerbral concussion（MCC）in rats.Methods： Forty-two male SD rats were randomly divided into seven groups： control group,post-injury for 1-24 d groups which were duplicated using a metallic pendulum-striker concussive device（n=6 for each group）.The expression of TPH in serotoninergic neuron in the basal forebrain and brainstem was detected and quantitated by image analysis system after immunohistochemical staining.Results： The expression of TPH was significantly increased in above brain areas at 2-8 d after the establishment of MCC（P0.05）,it decreased nearly to the normal level at 24 d（P0.05）.Conclusion： The expression of TPH in serotoninergic neurons is up-regulated following MCC,which could be related to the excitability of neurons after injury.