DC-CIK细胞协同索拉菲尼对肝癌细胞的杀伤作用

In vitro cytotox icity effects of cocultured DC-C IK cells combined with sorafenib against hepa to cellular carcinoma

目的:观察DC-CIK共培养细胞(DC-CIK)联合索拉菲尼(sorafenib)对肝癌细胞BEL-7402的体外杀伤效应。方法:取健康人外周血单个核细胞,加入不同细胞因子促进DC及CIK细胞成熟后混合共培养。MTT法检测用药后BEL-7402细胞增殖的情况CCK8试剂盒检测DC-CIK共培养细胞联合索拉菲尼对BEL-7402细胞的体外杀伤效应,Annexin V-FITC试剂盒检测两者联合对肝癌细胞凋亡率的影响。结果:索拉菲尼与DC-CIK细胞联合在一定浓度及效靶比时表现出对肝癌细胞的抑制作用明显增强,其中以索拉菲尼20.8μmol/L联合DC-CIK效靶比40∶1时杀伤率最高,达(72.24±2.42)%,是DC-CIK组的1.8倍,是索拉菲尼单药组的2.13倍,是CIK组的1.6倍(P<0.01)。索拉菲尼与DC-CIK细胞联合组诱导肝癌细胞凋亡率最高,达(77.36±1.92%(P<0.05)。结论:DC-CIK共培养细胞联合索拉菲尼在体外可显著抑制肝癌细胞的生长,分子靶向治疗联合细胞免疫治疗可能成为肝癌综合治疗的方法之一。

AIM： To investigate the in vitro inhibitory effects of DC（dendritic cell）-CIK（cytokine-induced killer cell） cocultured cells combined with sorafenib against hepatocellular carcinoma cell line BEL27402.METHODS： DC and CIK cells were generated in vitro by stimulating human peripheral blood mononuclear cells with different cytokines,and then they were cocultured.The cytotoxicity of DC-CIK cocultured cells（DC-CIK） combined with sorafenib against BEL-7402 cells was determined by CCK8 kit.The apoptosis of BEL27402 cells was measured by Annexin V-FITC Kit.RESULTS： The cytotoxicity rate of BEL27402 cells in DC-CIK ＋sorafenib group was significantly higher than those in the other there groups,with cytotoxicity rate in DC-CIK ＋ sorafenib group being（72.24±2.42）%,which was 1.8 times that in DC-CIK group,2.1 times that in sorafenib group,and 1.6 times that in CIK group（P〈0.01）.The apoptosis rate of BEL-7402 cells in DC-CIK ＋ sorafenib group was significantly higher than those in the sorafenib and DC-CIK groups,with the apop tosis rate in DC-CIK＋sorafenib group being（77.36±1.92）%（P〈0.05）.CONCLUSION： DC-CIK cocultured cells combined with sorafenib can inhibit the growth of hepatocellular carcinoma cell line BEL-7402 in vitro.Molecular targeting therapy combined with immunotherapy may be a new way for the comprehensive treatment of hepatocellular carcinoma.