HPLC法测定镉、砷胁迫下水稻根系的植物螯合肽

HPLC assay of phytochelatins in rice root under cadmium or arsenic stress

利用monobromobimane衍生试剂,通过反相高效液相色谱-荧光检测体系对镉、砷胁迫下水稻根系内的植物螯合肽(PCs)等巯基化合物进行形态和含量的分析测定.结果表明,采用乙腈和0.1%三氟乙酸组成的两元梯度流动相,可以将标准样品中的谷胱甘肽(GSH)和PCs等巯基化合物很好地分离.利用上述方法,能较好地分离Cd、As处理下溶液培养的水稻幼苗根系内的PCs形态.Cd处理下,根系内合成PC2、PC3、PC4,其中以PC3的含量最大.As处理下,PCs的形态除PC2和PC3外,其它PCn的形态有待进一步定性.

Phytochelatins（PCs） and other thiol-containing compounds in rice roots exposed to cadmium（Cd） and arsenic（As） were analyzed by RP-HPLC separation and fluorescence detection with monobromobimane pre-column derivatization.The results showed that GSH and PCs in the mixed standard solutions and rice roots can be separated by using ACN-0.1% TFA binary gradient elution.PC2,PC3 and PC4 were found in the rice roots that were exposed to Cd,and the concentrations of PC3 were the highest.In response to As stress,PCs in rice roots were complicated.In addition to PC2 and PC3,there are other forms of PCn that need further identification.