非小细胞肺癌患者外周血循环内皮干细胞的检测及其临床意义

Detection of circulating endothelial progenitors cells in peripheral blood in human non-small cell lung cancer

目的探讨外周血循环内皮祖细胞(endothelial progenitors cells,EPCs)与非小细胞肺癌患者的关系及临床意义。方法选择非小细胞肺癌患者45例和对照组15例,用抗CD133和KDR的单克隆抗体标记外周血细胞,应用流式细胞术检测其外周血中循环内皮祖细胞。应用Real-time RT-PCR方法检测患者外周血中CD133和KDR的mRNA表达水平。密度梯度离心法从人外周血分离出单个核细胞,将其接种在人纤维粘连蛋白包被培养板,在加有EGM-2-MV-SingleQuots的培养液中培养,在荧光显微镜下进行鉴定。其中U EA-1和D iI-A c-LDL染色双阳性细胞为正在分化的内皮祖细胞。结果肿瘤患者外周血中EPCs含量高于正常对照组(P<0.01),外周血中CD34和KDR的表达水平明显高于正常对照组。结论非小细胞肺癌患者外周血EPCs明显增高,为今后将EPCs作为非小细胞肺癌患者辅助诊断和治疗效果检测的标志物奠定基础。

Objective To verify Whether circulating endothelial progenitor cells（EPCs） participate in the progression of non-small cell lung cancer（NSCLC）.Methods EPCs labeled with CD133 and vascular endothelial growth factor receptor-2（VEGFR2） antibodies were counted by flow cytometry in 45 patients with NSCLC.By means of a real-time quantitative RT-PCR method,we measured VEGFR2,CD133,mRNA in the peripheral blood samples of the same patient population.Results We testify the existing of CEPs in peripheral blood.CEPs in patients with NSCLC were significantly increased compared with health control,and the markers for CEPs were also increased.Conclusion The amount of CEPs increased in patients with NSCLC.The EPCs can be used as a marker to monitor the efficacy of standard therapies in NSCLC.