eNOS基因单核苷酸多态性与2型糖尿病合并高血压相关性的研究

Association between eNOS gene single nucleitide polymorphism and Type 2 diabetes with hypertension

目的探讨吉林地区汉族人内皮型一氧化氮合酶(eNOS)基因第7外显子单核苷酸(G894T)多态性与糖尿病合并高血压的关系。方法对35例2型糖尿病合并高血压的患者,45例单纯2型糖尿病患者,36名健康人进行对照研究。抽提人外周血中白细胞的基因组DNA应用聚合酶链反应限制片段长度多态性(PCR-RFLP)法测定eNOS基因G894T多态性,将PCR产物酶切后进行2%的琼脂糖凝胶电泳,在紫外线灯下观察荧光带并确认每例的基因型。各组间的基因型频率比较应用Hardy-Weinberg遗传平衡定律和χ2检验,等位基因频率比较应用χ2检验。结果 (1)研究对象具有群体代表性。(2)eNOS基因的第7外显子单核苷酸(G894T)多态性位点:DM+HP组GT基因型频率和等位基因T频率显著高于NC组(χ2=11.188,P<0.05;χ2=4.807,P<0.05),DM组GT基因型频率和等位基因频率与DM+HP组与NC组无明显差异(χ2=1.923 5.518,P>0.05;χ2=1.538 1.212,P>0.05)。结论 eNOS基因第7外显子894G→T多态性与糖尿病合并高血压具有相关性。T等位基因是2型糖尿病合并高血压的危险因素。

Objective To investigate the association between the single nucleitide polymorphism in exon 7（G894T） of the endothelial nitric oxide synthase（eNOS） and type 2 diabetic patiens with hypertension in the Han population in jilin region.Methods This research is divided into research object totally 3 sets.The first group（A group） is the diabetic patiens without hypertension including 45 persons）;The second group（B group） is the diabetic patiens with hypertension including 35 persons;The second group（NC group） is matched control including 36 persons;Carry on 3 sets to check contrast control study.The next step is abstracting template DNA,adopt the American Promega company produce of the DNA extract kit quickly abstracting persons white cell DNA of peripheral blood.The G894T polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） analysis.the last step is 2% agarose gel electrophoresis was performed,the results were observed under ultraviolet light.Results（1）Hardy-Weinburg inherit quilibrium laws examination result is that each genotype frequency attains heredity balance and have a community representative.（2）The frequency of T allele and TG genetype at exon 7 of eNOS were significantly higher in the DM with hypertension group than that in matched control group;but there was not have no obvious difference between DM without hypertension and DM with hypertension.the result of DM without hypertension and matched control is the same.Conclusion The 894G→T polymorphism at exon 7 of eNOS gene may be a predisposal factor for diabetic with hypertension in jilin.T allele is related to diabetic with hypertension in type-2 diabetes mellitus.