体外人工肠液激活与未激活的猪带绦虫六钩蚴差异蛋白质组学比较

Proteomic analysis of the nonactivated and activated proteins of Taenia solium oncosphere stimulated by artificial intestinal fluid

为建立一种用于比较猪带绦虫六钩蚴(Taenia solium)激活前后蛋白差异表达的二维液相色谱方法,本研究对人工肠液激活前后的六钩蚴进行一维色谱聚焦和二维反相色谱分析,以0.3pH为单位在pH7.71～pH5.31的范围内梯度分离出208个激活和197个未激活的六钩蚴蛋白。Mapping tools软件分析结果表明,六钩蚴激活前后的差异蛋白数量为77个,其中激活的六钩蚴差异蛋白44个,未激活的六钩蚴差异蛋白33个。该方法具有分辨率高、重现性好、自动化程度高的特点,为研究六钩蚴与其宿主间的寄生机制提供研究方法。

For proteomic analysis of Taenia solium oncosphere,the one-dimensional chromatofocusing and two-dimensional liquid reversion phase chromatography was used to separate and compare the difference of protein expressions in nonactivated and activated of T.solium oncospherein stimulated by artificial instestinal fluid.Total of 208 activated and 197 nonactivated oncospherein proteins were obtained by 0.3pH gradient separation between pH7.71 to pH5.31.Seventy-seven differentially proteins were identified by Mapping tools comparison,of which 44 differentially expressed proteins were found in activated and 33 in nonactivated T.solium oncospherein.The analysis method was proved to be effective,stable and high-automaticity,which has tremendous developing potentiality on the research of differential proteomic expression profile.The research work sets up a foundation for the research on the parasite mechanism of host and oncosphere.