中国汉族人群GST1 GSTM1基因多态性与血脂水平及冠心病的相关性研究

Association of glutathione S - transferase T1 and M1 gene polymorphisms with lipoprotein levels and coronary heart disease in the Chinese Han population

目的通过研究中国汉族人群谷胱甘肽硫转移酶T1、M1（glutathione S—transferase，GSTF1、GSTM1）基因多态性与衄脂水平及冠心病（coronary heart disease，CHD）之间的关系，探讨冠心病的遗传易感性。方法采用病例一对照研究的方法，选取255例冠心病患者和与之年龄、性别相匹配的145例健康人作为对照组，采用聚合酶链式反应（polymerase chain reaction，PCR）方法检测GSTT1、GSTM1基因多态性，同时检测各研究对象的空腹血清总胆固醇（TC）、甘油三酯（TG）、载脂蛋白A（apoA）、载脂蛋白B（apoB）、高密度脂蛋白（HDL）、低密度脂蛋白（LDL）、脂蛋白a（Lpa）等临床资料。结果冠心病组GSTT1、GSTM1、GSTT1／GSTM1阳性基因型频率低于对照组（P=0．007、P=0．003、P=0．005），GSTT1／GSTM1阴性基因型频率高于对照组（P=0．001）。GSTM1阴性基因组apoB、LDL水平高于GSTM1阳性基因组（P=0．005、P：0．009）；GSTF1／GSTM1阴性基因组apoB、LDL水平高于GSTT1／GSTM1阳性基因组（P=0．014、P=0．013）。结论GSTT1和GSTM1阴性基因型可能是中国汉族人群冠心病的遗传易感基因，GSTM1基因多态性可能通过影响血脂代谢而参与冠心病的发病过程。

Objective To investigate the genetic susceptibility to coronary heart disease（CHD） by studying the association of glutathione S - transferase T1 and M1 （GSTT1, GSTM1 ） gene polymorphisms with lipoprotein levels and CHD in the Cilinese Han population. Methods In this case -control study, the GSTT1, GSTMI gene polymorphisms were detected by polymerase chain reaction （PCR） method in 255 CHD patients and age - and sex - frequencies matched 145 controls. Clinical parameters such as fasting serum total cholesterol （TC）, triglyceride （TG） , apolipoprotein A （apoA）, apolipoproteln B （apoB）, high - density lipoprotein （HDL）, low - density lipoprotein （LDL）, lipoprotein a （Lpa）, fasting blood glucose and other clinical data were measured. Results （1）The frequencies of GSTT1, GSTM1, GSTT1/GSTM1 positive genotype were lower in CHD group than in control group （P = 0. 007, 0. 003, 0. 005, respectively） and the frequencies of GSTT1/GSTMI negative genotype were higher in CHD group than in control group （P =0. 001 ）. （2）The levels of apoB and LDL were higher in GSTM1 negative genotype group than in GSTM1 positive genotype group （ P = 0. 005, 0. 009, respectively）, the indicators were higher in GSTT1/GSTM1 negative genotype group than in GSTT1/GSTM1 positive genotype group in all subjects （P =0. 014, 0. 013, respectively）. Conclusion GSTI＇I and GSTM1 negative genotypes are genetic susceptibility factors of coronary heart disease in Chinese Han population , GSTM1 gene polymorphisms may be involved in the pathogenesis of coronary heart disease by influencing the lipid metabolism .