程序性细胞死亡因子4蛋白表达与喉鳞状细胞癌相关性研究

Expression of programmed cell death 4 protein is closely correlated with laryngeal squamous cell carcinomas

目的:探讨程序性细胞死亡因子4(PDCD4)与喉鳞状细胞癌发生和发展的关系。方法:免疫组织化学方法和Western印迹法检测喉鳞状细胞癌组织及癌旁正常喉黏膜组织中PDCD4蛋白表达。结果:免疫组织化学染色显示喉鳞状细胞癌组织中PDCD4蛋白阳性表达率低于癌旁正常喉黏膜组织,差异有统计学意义(P<0.01)。喉鳞状细胞癌组织中PDCD4蛋白阳性表达和患者的年龄、性别、临床分型(肿瘤部位)、TNM分期无相关性,与病理分级、淋巴结转移有显著相关性。免疫印迹结果亦显示喉鳞状细胞癌组织中PDCD4蛋白表达低于癌旁正常喉黏膜组织(P<0.01)。结论:PDCD4蛋白低表达与喉鳞状细胞癌的发生和发展有关,PDCD4可能成为预测喉鳞状细胞癌转移和预后的生物学指标。

Objective：To study whether programmed cell death 4（PDCD4） is involved in the development and progression of laryngeal squamous cell carcinomas（LSCC）.Method：The expressions of PDCD4 protein were examined by immunohistochemical staining and Western blot in LSCC tissues and their surrouding laryngeal normoal mucosa tissues.Result：The positive rate of PDCD4 protein in LSCC was significantly lower than that in the laryngeal normoal mucosa tissues by immunohistochemical staining（P0.01）.No correlation was found between positive expression of PDCD4 and gender,age,tumor site,TNM stage,but there was significant correlation between positive expression of PDCD4 from differentiation grading and lymphatic matastasis.Meanwhile,we got the similar results by Western blot（P0.01）.Conclusion：The low expression of PDCD4 protein is closely related to the development and progression of LSCC.PDCD4 might be a novel molecular marker for prognosticating metastases and prognosis of LSCC.