白癜风患者血清及皮损疱液中白细胞介素-22水平测定

Cytokine level mensuration of IL-22 in serum and skin blister fluid with vitiligo patients

目的检测白癜风患者血清及皮损疱液中白细胞介素-22（interleukin-22，IL-22）水平，分析处于不同病期及经治疗后不同疗效的白癜风患者血清IL-22水平变化情况，探讨该因子在白癜风发病过程中的可能作用。方法采用酶联免疫吸附测定法对56例患者、20例健康志愿者血清及15例接受表皮移植患者皮损疱液中IL-22因子水平进行测定，对接受治疗的18例患者血清中该因子水平进行监测，采用SPSS13．0软件进行数据分析。结果进展期、稳定期、对照组血清中IL-22水平依次降低，且两两组问差异均有统计学意义（P〈0．01），接受表皮移植患者皮损区疱液中IL-22含量显著高于供皮区水平，差异有统计学意义（P〈0．05）。18例疗效较好患者血清中IL-22水平与治疗前比较下降明显，差异有统计学意义（P〈0．05）。疗效较差患者血清中IL-22水平差异无统计学意义（P〉0．05）。结论IL．22可能在白癜风发病及加重过程中起重要作用，IL-22水平变化可能成为预测白癜风患者病情进展及评价疗效的指标之一。

Objective To detect the serum and suction blister fluid IL-22 level in vitiligo patients, to analyze the change of serum IL-22 level in different stages and different therapeutic effects, and to explore the possible role of IL-22 in pathogenesy leading to vitiligo. Methods The serum IL-22 level in 56 patients and 20 healthy control, and those in blister fluid of 15 cases treated by epidermic grafting were examined by the ELISA methods. The serum IL-22 level in 18 subjects who accepted the whole range combined therapy were monitored. All the data were analyzed by SPSS 13.0. Results The serum IL-22 level of active phase, stable phase and controls degrade gradually, and the interclass difference in both groups were statistically significant （ P〈0.01 ）. The IL-22 level of skin lesions blister fluid were significantly higher than those of normal skin in cases treated by epidermic grafting （P〈0.05）. The serum IL-22 level of 18 cases who have better effect decreased significantly than those before treatment （ P 〈 0. 05 ）, but the cases who have worse effect were not statistically difference （ P 〉 0. 05）. Conclusion IL-22 might play an important role in the development and aggravation of vitiligo, and it could be the index of predicting pathogenetic condition and evaluating curative effect of vitiligo patients.