外用他克莫司小鼠－大鼠异种触须毛囊移植

Topical administration of tacrolimus prolong the survival of xenotransplantion of the hair follicles from mouse to rats

目的探讨建立小鼠一大鼠异种触须毛囊移植动物模型的可行性及局部辅助应用他克莫司的效果。方法以C57BL／6J小鼠触须毛囊为供体、Wister大鼠为受体，进行异种毛囊移植。实验分两组：移植后局部外用他克莫司组（A组），移植后的空白对照组（B组）。观察移植后毛囊的成活时间及形态学变化。结果A组的毛囊最长成活15d，B组的毛囊最长成活10d。两组比较表明，他克莫司延长了异种移植毛囊的成活时间，但触须毛囊含血窦，易诱发免疫排斥反应。结论本动物模型是一种研究异种毛囊移植和免疫赦免的良好动物模型。外用他克莫司可以延长异种移植触须毛囊的成活时间，但仅靠局部用药仍无法避免排斥反应的发生。

Objective To study the establishment of xenotransplantation of vibrissa follicles in the mouse-to-rats mode and the effect of topical tacrolimus on the survival of the xenotransplantated hair follicles. Methods In our study, vibrissa follicles of C57BL/6J mice were used as donor and Wister rats were used as recipients. We harvested the black vibrissa follicles of C57BL/6J inbred mice, then transplanted them into white Wister rats dorsal. Totally 20 couples of rats were divided in 2 groups ： topical taerolimus group （ Group A ） , blank control group （ Group B）. Then we compared the survival time of vibrissa follicles and their histologie outcomes to verified the practicability of xenogeneic transplantion of vibrissa follicles, and the topical application of tacrolimus results postoperatively. Results The longest survival time was 15 days in Group A, and 10 days in Group B, respectively. It could be seen by comparing, topical therapy with taerolimus could prolong the survival time of the xenograft temporarily. The blood cells in the vibrissa follicle sinus could induce immunological rejection. Conclusion It is a successful model to study xenotransplantation of vibrissa follicles and immune privilege. Topical administration of tacrolimus prolong the survival of the xenogeneic transplanted vibrissa follicles in rats.