不同环境中嗜肺军团菌FlaA和CsrA基因表达水平初步研究

Preliminary Study on Differential Expression of FlaA and CsrA Gene of Legionella pneumophila in Different Environmental Media

目的初步探讨多种环境介质中嗜肺军团菌FlaA和CsrA基因表达水平的差异。方法于2010年8月和9月随机选取南京市四家公共场所采集集中空调系统的冷却水、冷却塔旁土壤、风管内壁积尘和室内送风口气溶胶,喷泉水、喷泉旁土壤,宾馆淋浴水样品。采用实时荧光定量PCR方法检测环境样本中嗜肺军团菌FlaA和CsrA基因的表达水平。结果不同环境样本中嗜肺军团菌FlaA和CsrA基因均有表达,淋浴水中FlaA基因的相对表达量上调6.798 4倍,CsrA基因相对表达量上调1.483 7倍。结论不同环境介质中嗜肺军团菌FlaA和CsrA基因的表达水平有变化,FlaA基因的总体表达水平稍高于CsrA基因。

Objective To explore differential expression F/aA and CsrA gene of LegioneUa pneumophila in different environmental media. Methods The environmental samples collected from four public places of Nanjing in Aug. and Sep., 2010,were detected by real-time PCR and analyzed by 2 methods. Results The expression of FlaA and CsrA gene of Legionella pneumophila varied with different environmental media. The relative expression of FlaA and CsrA gene of Legionella pneumophila in bathing water increased by 6.798 4 and 1.483 7 times respectively. Conclusion The expression of FlaA and CsrA gene of Legionella pneumophila can be influenced by environmental media.