HPLC测定“冬病夏治”贴膏中延胡索乙素的含量

Determination of tetrahydropulmatine content in treatment of winter's diseases in summer path by HPLC

目的：建立高效液相色谱法测定＂冬病夏治＂贴膏中延胡索乙素含量的方法。方法：采用Hypersil ODS2色谱柱（4.6 mm×250 mm,5μm）;流动相甲醇-0.1%磷酸溶液（三乙胺调pH6.28）（63∶37）;流速1.0 mL·min-1;检测波长280 nm。结果：延胡索乙素线性范围2.08~49.92 mg·L-1（r=0.999 8）,平均回收率和RSD分别为97.85%,2.49%。结论：方法简便、可靠,可用于＂冬病夏治＂贴膏中延胡索乙素含量测定。

Objective： To establish a high performance liquid chromatography method for the determination of tetrahydropulmatine content in treatment of winter′s diseases in summer path.Method： The analysis was performed on the Hypersil ODS2 column（4.6 mm×250 mm,5 μm） with methanol and 0.1% phosphoric acid solution（adjusted pH to 6.28 with triethylamine） as mobile phase at 63∶37.The flow rate was 1.0 mL·min-1 and the UV detection wavelength was 280 nm.Result： The linear range of tetrahydropulmatine was obtained from the range of 2.08-49.92 mg·L-1（r=0.999 8）.The average recovery was 97.85% with RSD 2.49%.Conclusion： This method is simple,reliable and can be used for the determination of tetrahydropulmatine in this preparation.