UPLC-MS分析侧柏叶中黄酮类化合物

Analysis of flavonoids in Platycladi Cacumen by UPLC-MS

目的:采用超高效液相色谱-质谱仪联用技术(UPLC-MS)对侧柏叶中黄酮类成分进行分析和鉴别。方法:用Waters BEHC18柱(50 mm×2.1 mm,1.7μm)。流动相为0.2%甲酸水溶液-甲醇梯度洗脱,使用ESI离子源,在负离子模式下采集数据。结果:推断出侧柏叶中11个黄酮类化合物,并探讨了黄酮类化合物的电喷雾/串联质谱(ESI-MS-MS)的裂解方式。结论:经过超高效液相色谱的分离,利用质谱测定提供的准确质量数,结合紫外光谱的信息可以鉴定侧柏叶中主要的黄酮类成分。探讨了黄酮类化合物的电喷雾/串联质谱(ESI-MS/MS)的裂解方式,为其成分鉴定提供了准确有效的方法。

Objective： To investigate the flavonoids in Platycladi Cacumen.Method：The constituents in Platycladi Cacumen were determined by UPLC-MS.A Waters BEH C18column（2.1 mm×150 mm,1.7 μm） was used with a gradient elution of methanol-water containing 0.2% formic acid.The mass spectrometer equipped with electrospay ionization source was used as defector and operated in data was collected under the negative ion modes.Result： Eleven constituents were identified.Conclusion： In this study,the main flavonoids in Platycladi Cacumen were separated by UPLC,and identified through the information of mass number and UV spectra.With the information of MS/MS from flavonoids,the modes of breaking up flavonoids were discussed.It is an accurate and effective method which can be applied for the constituent identification of Platycladi Cacumen.