茜素络合物浸泡标记秦岭细鳞鲑发眼卵及仔鱼耳石

Alizarin marking of otolith at eyed eggs and larvae stages in Brachymystax lenok tsinlingensis

采用茜素络合物(ALC)在秦岭细鳞鲑(Brachymystax lenok tsinlingensis)发眼卵和仔鱼阶段进行浸泡标记试验,为确定合适浸泡浓度和持续浸泡时间,试验设置了6个的浓度组和4个时间梯度组。结果显示:在荧光显微镜下观察被浸泡的仔鱼和经浸泡处理的发眼卵而出膜后的仔鱼,其耳石都出现橘红色荧光标记。浸泡发眼卵组最适处理条件是茜素络合物溶液浓度30 mg/L持续浸泡18 h,标记率100%,孵化率91%,出膜后15 d仔鱼存活率91%,标记环"非常明显";仔鱼浸泡组最适处理条件是茜素络合物溶液浓度120 mg/L持续浸泡12 h,标记率83.3%,处理15 d后仔鱼存活率92.3%,相比对照组统计检验无差异(P<0.05),标记环"较明显"。相比上述两种方法,浸泡发眼卵试验组相比浸泡30日龄仔鱼试验组所需要的茜素络合物(ALC)溶液浓度更低,而且在标记成功率、标记效果、标记后仔鱼存活率以及标记成本等方面也更为理想。因此,用茜素络合物(ALC)在秦岭细鳞鲑发眼卵阶段进行荧光标记是可行的,这种方法也可在其它鱼类标记中尝试,同时将人工繁育鱼苗与野生鱼苗加以区别,有利于开展人工增殖放流效果及早期生活史等研究。

To study the effects of ALC for marking the eggs and larvae,Brachymystax lenok tsinlingensis eyed eggs and larvae were immersed with twenty-four combinations of six different ALC concentration and four treatment time.The otoliths of larvae were detected by fluorescence microscope.The results showed that the larvae immersed in alizarin complexone solution or which from treated eggs presented distinct scarlet-pink fluorescent marks on otolith under UV light.30 mg/L for 18 h was found to be the most appropriate for eyed eggs with 100% of marking rate and 91% of hatching and larvae survival rate.The marking effect was ＂bright mark＂.120 mg/L for 12 h was the most appropriate for 30 days old larvae with 83.3% marking ratio and 92.3% larvae survival rate after treated for fifteen days,which had no statistically difference with that of the control group（P〉0.05）.The marking effect was ＂light mark＂.Compared with larvae group,eyed eggs group needed lower concentration of ALC,which was more ideal on success ratio of marking,marking effect,post-larvae survival rate and marking cost.This method for marking otoliths of B.lenok tsinlingensis with ALC during eyed eggs is recommended,which could be used to mark other fish.Furthermore,the method could be applied to distinguish hatchery-produced fish from wild fish,which is helpful to assessment of different stock enhancement and surveys of the early life history.