浸种催芽对打破云南红豆杉种子休眠的初步研究

Preliminary Study on the Seed Dormancy Breaking Effect of Germination Soaking Methods on Taxus yunnanensis

以云南红豆杉(Taxus yunnanensis Cheng et L.K.Fu)种子为研究对象,将种子浸种催芽处理后在基本培养基上培养,探索能有效解除种胚休眠时间过长的途径。结果显示,云南红豆杉种子具有内生菌;离体培养条件下种子较易形成愈伤组织,表明种子有足够的内源激素;尽管各浸种方法均不能有效打破种子休眠、促进萌发,但发现细胞分裂素有促进愈伤组织形成的作用,赤霉素处理比清水处理的萌发率略高,这说明单一激素处理不能有效地解除种子休眠,可进一步做激素之间的交互作用以及对激素应答方面的研究。

The seeds of Taxus yunnanensis Cheng et L.K.Fu was used to study the effective method of releasing seed dormancy by soaking seeds in different solution.The results showed that some endogenous bacteria existed in the seeds of T.yunnanensis;the cultured seeds could produce callus easily which indicated that there were sufficient endogenous growth substances.Cytokinins had a little acceleration effect on the formation of callus though no soaking methods could release dormancy effectively;and gibberellin had slight effect on increasing the germination rate of the seeds.The results indicated that single treatmeat of any growth substance couldnot release the dormancy of T.yunnanensis seeds effectively;further study should be conducted to find out the action and interaction of growth substances.