小柴胡汤对病毒性心肌炎动物模型IL-2、TNFα、T淋巴细胞亚群的影响

Effects of Minor Bupleurum Decoction on the activation of T lymphocyte subsets and the production of interleukin 2 and tumor necrosis factor-alpha in myocarditis model

目的探讨小柴胡汤对病毒性心肌炎动物模型白细胞介素2（IL-2）、肿瘤坏死因子α（TNFα）和T淋巴细胞亚群的作用。方法将40只6-8周龄、体质量15-19 g雌性BALB/c小鼠随机分成治疗组和对照组,每组20只。经腹腔接种亲心肌细胞株柯萨奇病毒B3建立心肌炎模型,用小柴胡汤灌胃给药治疗。分别于治疗后5、7、15和21 d检测血清中的IL-2、INFα和血液中的T淋巴细胞亚群。结果病毒感染后5 d,治疗组小鼠IL-2活性无明显增高,与对照组比较差异无统计学意义;感染后7-21 d,治疗组小鼠IL-2活性逐渐增高,与对照组比较差异有统计学意义[7d：（83.4±4.6）mg/Lvs（72.7±4.2）mg/L,P〈0.05;15d：（86.7±5.1）mg/Lvs（69.4±4.5）mg/L;21d：（95.3±4.8）mg/Lvs（64.2±4.6）mg/L]。病毒感染后5-21 d,治疗组小鼠血清中TNFα水平逐渐增高,与对照组比较差异有统计学意义[5d：（158.8±11.3）mg/Lvs（87.2±13.7）mg/L,P〈0.01;7d：（173.7±12.5）mg/Lvs（109.6±12.4）mg/L,P〈0.01;15d：（294.2±16.2）mg/Lvs（127.5±15.1）mg/L,P〈0.01;21d：（366.7±18.6）mg/Lvs（145.3±18.3）mg/L,P〈0.01]。病毒感染后5 d,治疗组小鼠外周血中CD4^＋和CD8^＋均无明显增高,与对照组比较差异无统计学意义;感染后7-21 d,治疗组小鼠外周血中CD4^＋和CD8^＋均逐渐增高,与对照组比较差异有统计学意义。结论小柴胡汤具有调节免疫应答功能,从而对病毒性心肌炎起到有效治疗作用。

Objective To explore the effects of Minor Bupleurum Decoction（MBD）on the activation of T cell subsets and the production of interleukin 2（IL-2） and tumor necrosis factor α（TNFα） in myocarditis model.Methods The model of myocarditis was established by intraperitoneally inoculated BALb/c female mice（age： 6-8 weeks,weight：15-19 g） with cosackievirus type B3.A total of 20 mice was randomly assigned into MBD treatment group and control groups.The mice of the MBD treatment group were intragastrically administrated with MBD once a day for 21 days,while the mice of the control group were simultaneously treated with physiological saline solution.Results There were no significant differences in the levels of serum IL-2 and the lymphocyte subsets of CD4^＋ and CD8^＋ in 5 days after treatment between the two groups.The levels of IL-2 were significantly higher in the MBD treatment group than in the control group on day 7[（83.4±4.6）mg/L vs（72.7±4.2）mg/L,P〈0.05],day 15[（86.7±5.1）mg/L vs（69.4±4.5）mg/L,P0.01]and day 21[（95.3±4.8）mg/L vs（64.2±4.6）mg/L,P0.01]of the treatment.The levels of serum TNFα were significantly higher in the MBD treatment group than in the control group on day 5[（158.8±11.3）mg/L vs（87.2±13.7）mg/L,P〈0.05],day 7[（173.7±12.5）mg/L vs（109.6±12.4）mg/L,P0.01],day 15[（294.2±16.2）mg/L vs（127.5±15.1）mg/L,P0.01] and day 21[（366.7±18.6）mg/L vs（145.3±18.3）mg/L,P0.01]of treatment.The percentages of CD4^＋ and CD8^＋ T lymphocytes were also significantly higher in the MBD treatment group than in the control group after 7 days of treatment（P〈0.05）.The pathological examination of cardiovascular tissue suggested that the damages of myocarditis were milder in the mice of the MBD treatment group than in the mice of the control group.Conclusions MBD can regulate the function of the immune response,and thus play an important role in the effective treatment of viral myocarditis.