高效液相色谱法同时测定冰淇淋中的10种合成色素

Simultaneous Determination of Ten Synthetic Colours in Ice-cream by High Performance Liquid Chromatography

建立了一种同时测定冰淇淋中靛蓝、新红、亮蓝、柠檬黄、日落黄、苋菜红、胭脂红、偶氮玉红、诱惑红、赤藓红10种合成色素的高效液相色谱分析方法。对提取试剂、流动相条件、最大吸收波长进行了研究，确定了最佳提取方法及色谱条件。待测样品经甲醇-水提取，加水稀释后上机分析，采用Symmetry C18柱（25cm×4．6mm×5μm），以甲醇-20mmol／L醋酸铵为流动相，在最佳梯度洗脱条件下对10种合成色素进行分离，二极管阵列检测器检测，外标法定量。结果表明，各组分在0．5～10．0mg／L范围内呈良好线性，相关系数（r）均大于0．997，在2、10、50mg／kg3个加标水平下的平均回收率为76％～108％，相对标准偏差（RSD）为0．56％～2．8％，检出限为0．2～1．0mg／kg。该方法简单、快速、灵敏、准确，可用于冰淇淋中10种合成色素的日常检测。

A high performance liquid chromatographic method was developed for the simultaneous determination of 10 synthetic eolours such as indigotin, new red, brilliant blue, tatrazine, sunset yellow, amaranth, poneeau 4R, carmoisine, allura red and erythrosine in ice-cream. The optimal extraction conditions and HPLC chromatographic conditions were confirmed by investigating the extracting solvent, mobile phase type and maximum absorption wavelength. The sample was extracted with methanol water solution, and the extract diluted with water was separated on a Symmetry C18 （25 cm ×4.6 mm ×5 μm） column using methanol - 20 mmol/L ammonium acetate as mobile phase by gradient elution. The target compounds were confirmed by diode-array detection and quantified by the external standard method. Under the optimal conditions, the calibration curves of 10 synthetic colours showed good linearities in the range of 0.5 - 10.0 mg/L, with their correlative coefficients more than 0. 997. The mean recoveries at 3 spiked levels of 2, 10, 50 mg/kg ranged from 76% to 108% with RSDs of 0.56% - 2. 8% . The detection limits were in the range of 0.2 - 1.0 mg/kg. The method was simple, rapid, sensitive and accurate, and was suitable for the determination of 10 synthetic colours in ice-cream.