高效毛细管电泳安培法测定肉苁蓉中松果菊苷的含量

Determination of Echinacoside in Cistanche deserticola Y.C.Ma by Capillary Electrophoresis with Amperometric Detection

采用毛细管电泳安培法建立了肉苁蓉中松果菊苷含量的检测方法，探讨了缓冲溶液种类、工作电位、分离电压、进样时间等对分离和检测的影响。在15mmol／L硼砂缓冲溶液（pH9．5），25kV电压，0．6V工作电位的条件下，松果菊苷的质量浓度在0．5—50．0mg／L范围内与峰面积呈良好线性，相关系数为0．9994，检出限（S／N=3）为0．1mg／L，回收率为98％-102％。该法简便、快速、准确，可用于肉苁蓉中松果菊苷的质量控制。

A capillary electrophoresis method coupled with amperometric detection （CE/AD） was developed for the determination of echinacoside in Cistanche deserticola K C. Ma. Effects of types and pH values of running buffer, detection potential, applied voltage and sampling time on separation efficiency were investigated. The optimized conditions were as follows： running buffer： 15 mmol/L Na2B4O7 buffer（pH 9.5） , separation voltage： 25 kV, detection potential： 0.6 V. Under the optimal conditions, a good linearity was obtained between peak area and mass concentration of echinacoside in the range of 0.5 - 50.0 mg/L with a correlation coefficient of 0. 999 4. The detection limit （S/N = 3） was 0. 1 mg/L and the spiked recoveries ranged from 98% to 102% . The method is simple, rapid and accurate, and can be applied to control the content of echinacoside in Cistanche deserticola Y. C. Ma.