摘要
目的研究激光原位角膜磨镶术(LASIK)术后干眼患者结膜印迹细胞学(IC)检查的意义。方法研究接受LASIK手术25位患者50只眼,采用球结膜印迹细胞学检查联合PAS染色,观察术前球结膜杯状细胞数量及结膜上皮细胞形态学改变。结果术后7例患者14只眼表现为干眼组(CDEG)。观察5个高倍视野的杯状细胞数量,CDEG组6.07±2.12,明显减少,无干眼组(NDEG)103.83±9.51,P<0.01。CDEG组鳞状化生分级平均秩次31.64明显高于NDEG组的平均秩次23.11,P<0.05。结论 IC是一种无创、简便、准确的检查方法,球结膜杯状细胞减少及鳞状化生与LASIK术后干眼有关。
Objective To evaluate the effect of impression cytology（IC） in the chronic dry-eye after Laser in situ keratomileusis（LASIK）.Methods Impression cytology combined PAS staining were applied in 25 patients（50 eyes） after LASIK.Conjunctival epithelial cells and the number,morphology and changes of goblet cells were observed.Results 7 patients（14） eyes presented chronic dry-eye（CDEG）.The number of goblet cells was calculated using five visuaI fields randomly under high magnification.It was significant decreased in CDEG（6.07±2.12） compared with NDEG（103.83±9.51）（P〈0.01）.Eyes of CDEG demonstrated a significantly higher grade（31.64） of squamous metaplasia and lower goblet cell density compared with NDEG（23.11）（P〈0.05）.Conclusion Impression cytology represents a non-or minimally invasive biopsy of the ocular surface epithelium with no side effects or contraindications.Higher grade of squamous metaplasia and lower goblet cell density related with chronic dry-eye after LASIK.
出处
《宁夏医学杂志》
CAS
2011年第6期481-482,共2页
Ningxia Medical Journal
基金
宁夏自然科学基金资助项目(NZ08133)
