期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

肉豆蔻提取物对小鼠小胶质BV2细胞环氧合酶2表达的影响 被引量：3

Effects of myristica fragrans extracts on expression of cyclooxygenase 2 in murine microglial BV2 cells
下载PDF
导出
摘要 目的探讨肉豆蔻提取物对小鼠小胶质BV2细胞的作用机制。方法运用WST-8法检测肉豆蔻提取物对小鼠小胶质BV2细胞生存率的影响,倒置显微镜观察其形态学改变,Western Blot检测脂多糖(LPS)所诱导的小鼠小胶质细胞环氧合酶2(COX-2)的表达。结果肉豆蔻提取物对小鼠小胶质BV2细胞无明显毒性;可抑制谷氨酸对小鼠小胶质BV2细胞的形态学损害;抑制LPS所诱导的小鼠小胶质BV2细胞COX-2的表达。结论肉豆蔻提取物对小鼠小胶质BV2小胶质细胞具有保护作用。 Objective To study the mechanism of myristica fragrans extracts on murine microglial BV2 cells.Methods WST-8 method was used to detect the effect of myristica fragrans extract on viability rate of murine microglial BV2 cells,inverted microscope was employed to observed its morphological changes and Western Blot was conducted to measure the expression of cyclooxygenase 2（COX-2） in murine microglial BV2 cells induced by lipopolysaccharide（LPS）.Results Myristica fragrans extracts showed no sign of obvious toxicity on murine microglial BV2 cells,but it could inhibit glutamic acid caused damage to murine microglial BV2 cells and inhibit expression of COX-2 in murine microglial BV2 cells induced by LPS.Conclusion Myristica fragrans extracts show a protective effect on murine microglial BV2 cells.
作者 崔春爱 毛翘 金德山 张庆镐
机构地区 延边大学基础医学院
出处 《重庆医学》 CAS CSCD 北大核心 2011年第17期1731-1732,1735,共3页 Chongqing medicine
基金 吉林省教育厅(2008第19号) 延边大学资助项目(2008年)
关键词 小神经胶质细胞 环加氧酶 肉豆蔻提取物 谷氨酸 脂多糖类 microglia cyclooxygenase myristica fragrans extracts glutamic acid lipopolysaccharides
分类号 R285.5 [医药卫生—中药学]
  • 相关文献

参考文献15

  • 1Politis M,Pavese N,Tai YF,et al. Microglial activation in regions related to cognitive {unction predicts disease onset in Huntington's disease:A multimodal imaging study[J]. Hum Brain Mapp,2011,32(2) :258-270.
  • 2Hamada S, Koga T, Ooshima T. Virulence factors of Streptococcus mutans and dental caries prevention[J]. J Dent Res, 1984,63(3) : 407-411.
  • 3Loesche WJ. Role of Streptococcus mutans in human dental decay[J]. Mierobilo Rev, 1986,50 (4) : 353-380.
  • 4Cui CA,Jin DQ, Hwang YK, et al. Macelignan attenuates LPS-induced inflammation and reduces LPS-induced spatial learning impairments in rats[J]. Neurosciecne Letters,2008,448(1) :110-114.
  • 5Jin DQ,Lim CS,Hwang JK,et al. Anti-oxidant and anti-inflammatory activities of macelignan in murine hippocampal cell line and primary culture of rat microglial cells[J]. Biochem Biophys Res Commun,2005, 331(4) :1264-1269.
  • 6Ma J, Hwang YK, Cho WH, et al. Macelignan attenuates activations of mitogen-activated protein kinases and nuclear factor kappa B induced by lipopolysaccharide in microglial cells[J]. Biol Pharm Bull, 2009,32 (6):1085- 1090.
  • 7Akiyama H, Barger S, Barnum S, et al. Inflammation and Alzheimer's disease[J]. Neurobilo Aging, 2000,21 (3) : 383-421.
  • 8Vargas DL, Nascimbene C, Krishnan C, et al. Neuroglial activation and neuroinflammation in the brain of patients with autism[J]. Ann Neuol, 2005,57 (1) : 67-81.
  • 9Block ML, Zecca L, Hong JS. Microglia-mediated neurotoxicity: uncovering the molecular mechanisms [J]. Nat Rev Neurosci,2007,8(1) :57- 69.
  • 10Block ML, Hong JS. Microglia and inflammation mediated neurodegeneration: multiple triggers with mechanism[J]. Prog Neurobiol,2005,76(2) :77-98.

二级参考文献10

共引文献11

同被引文献31

  • 1王远志,李坤,贾天柱.肉豆蔻与长形肉豆蔻挥发油成分GC-MS比较分析[J].吉林医药学院学报,2008,29(2):85-87. 被引量：9
  • 2Block M L, Zecca L, Hong J S. Microglia mediated neurotoxicity: uncovering the molecular mechanisms [ J ]. Nat Rev Neurosci, 2007, 8:57-69.
  • 3Cui C A, Jin D Q, Hwang Y K, et al. Macelignan attenuates LPS-induced inflammation and reduces LPS-induced spatial learning impairments in rats[J].Neurosci Lett, 2008, 19, 448(1) :110-114.
  • 4Schoenborn J R, Wilson C B. Regulation of interferongamma during innate and adaptive immune responses [ J ]. Adv Immunol, 2007, 96:41-101.
  • 5Taubert A, Hermosilla C. Bovine recombinant IFN-γ induces endothelial cell gene transcription of immunoregulatory molecules and upregulates PMN and PBMC adhesion on bovine endothelial cells [J].Vet Res Commun, 2008, 32( 1 ) :35-47.
  • 6Politis M, Pavese N, Tai Y F, et ai. Microglial activation in regions related to cognitive function predicts disease onset in Huntington's disease[ J]. Hum Brain Mapp, 2011, 32(2) :258-270.
  • 7Brown G C, Bal-Price A. Inflammatory neurodegeneration mediated by nitric oxide, glutamate and mitochondria [J]. Mol Neurobiol, 2003, 27(3):325-355.
  • 8Chung J Y, Choo J H, Lee M H, et al. Anticariogenic activity of Macelignan isolated from Myristica fragrans against streptococcus mutans [ J ]. Phytomedicine, 2006, 13(4) :261-266.
  • 9Jin D Q, Lim C S, Sung J Y, et al. Ulva conglobata, a marine algae, has neuroprotective and anti-inflammatory effects in murine hippocammpal and microglial cells [ J ]. Neurosci Lett, 2006, 402(1/2):27-31.
  • 10Soares H, Waechter H, Glaichenhaus N, et al. A subset of dendritic cells induces CD4 + T cells to produce IFN- gamma by an IL-12-independent but CD70-dependent mechanism in vivo [ J ]. J Exp Med, 2007, 204 ( 5 ) : 1095-1106.

引证文献3

二级引证文献22

重庆医学
2011年 第17期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部