岩柏草总黄酮抗大鼠肝纤维化的实验研究

Empirical study of total flavonoids of selaginella moellendorfii hieron on hepatic fibrosis rat

目的:探讨岩柏草总黄酮对实验性肝纤维化大鼠的保护作用。方法:采用50%CCl4皮下注射8周,在造模同时分别灌胃15、30、60 mg/kg剂量岩柏草总黄酮混悬液,并以水飞蓟宾为实验对照。8周后处死大鼠,对大鼠肝脏纤维化评分,并检测血清和肝组织各指标。结果:CCl4造模结束后,大鼠肝组织病理结果显示其肝纤维化处于III-IV期;与模型组比较,岩柏草总黄酮能显著降低大鼠血清谷丙转氨酶(ALT)、谷草转氨酶(AST)和肝组织中丙二醛(MDA)水平,明显升高肝组织中超氧化物歧化酶(SOD)水平,显著改善大鼠肝纤维化的病理形态。结论:岩柏草总黄酮对CCl4诱导的大鼠肝纤维化有较好的保护作用。

AIM： To explore the protective effect of Selaginella moellendorfii Hieron on experimental hepatic fbrosis rat. METHODS： Hepatic fibrosis model was made by subcutaneous injection 50% carbon tetrachloride（CCl4） for 8 weeks,and then were intragastric administered with total flavonoids of Selaginella moellendorfii Hieron suspension（15,30,60 mg/kg）,silybin was experimental control group.All rats were killed after 8 weeks,the score of hepatic fibrosis and the index in liver tissue and serum were evaluated.RESULTS：The result showed that the hepatic fibrosis was in III-IV stage.Compared with the model group,total flavonoids of Selaginella moellendorfii Hieron could significantly decrease the levels of ALT,AST in serum,and the MDA in liver tissue,and increase the level of SOD.The pathomorphology of hepatic fibrosis rat was improved.CONCLUSION： Total flavonoids of Selaginella moellendorfii Hieron have a better protective effect on rat hepatic fibrosis induced by CCl4.