腹腔镜胃肠手术治疗2型糖尿病的血清蛋白质组学初步研究

Serum proteomic analysis after laparoscopic gastrointestinal surgery for the treatment of type 2 diabetes mellitus

目的探讨腹腔镜胃肠手术前后2型糖尿病患者血清蛋白质表达的差异。方法收集2008年6月至2010年9月第二军医大学附属长海医院行腹腔镜胃肠手术治疗的12例2型糖尿病患者的血清标本，双向凝胶电泳分离2型糖尿病患者术前、术后1周及术后1个月的血清总蛋白，对手术前后差异表达明显的蛋白点行质谱鉴定及生物信息学分析。结果建立了稳定、重复性好的血清凝胶蛋白图谱。筛选出2型糖尿病患者胃肠手术前后血清中差异表达明显的20个蛋白点，并成功鉴定出8个蛋白点，其中术后表达增高的有5个，分别是RhoGDP—dissociation inhibitor 1、Prohibitin、Alpha-1-antitrypsin precursor、Serotrans—ferrin precursor和Fibrinogen gamma chain precursor；术后表达降低的有3个，分别是MAP3K12-binding inhibitory protein 1、Coronin-1A和Isovaleryl-CoA dehydrogenase。结论2型糖尿病患者行腹腔镜胃肠手术后的血清蛋白质与术前比较，存在着明显的差异。共成功鉴定出8个蛋白点。

Objective To investigate the impact of laparoscopic gastrointestinal surgery on serum protein expression in patients with type 2 diabetes mellitus （T2DM）. Methods Twelve patients with T2DM received gastrointestinal surgery at Changhai Hospital of the Second Medical University from June 2008 to September 2010. Their serum samples were collected at different time points （before surgery, 1 week and 1 month after surgery）. Total proteins were seperated by two-dimensional （2D） gel eleetrophoresis. The differentially expressed proteins were analyzed by mass spectrometry and bioinformatics. Results Protein extracts of the serum samples were separated on 2D gels successfully. Twenty differentially expressed proteins in the serum after surgery were screened out. Eight proteins were successfully identified, in which the expression of 5 proteins （Rho GDP-dissociation inhibitor 1, Prohibitin, Alpha-l-antitrypsin precursor, Serotransferrin precursor and Fibrinogen gamma chain precursor） was increased after operation, and the expression of 3 proteins （ MAP3K12-binding inhibitory protein 1, Coronin-lA and Isovaleryl-CoA dehydrogenase） was decreased. Conclusions The expression of 20 proteins have been changed significantly in serum samples after laparoscopic gastrointestinal surgery in patients with T2DM, and 8 proteins were successfully identified.