摘要
目的:研究白藜芦醇体外活性,确定它的植物雌激素作用。方法:采用MTT法观察不同浓度白藜芦醇对MCF-7细胞增殖作用的影响。采用DNA ladder法和荧光显微镜观察高浓度白藜芦醇对细胞的影响。免疫组化法观察低浓度白藜芦醇对核增殖抗原PCNA表达的影响。结果:MTT结果显示白藜芦醇高浓度抑制MCF-7细胞增殖,IC50为8.70×10-5mol/L;低浓度(10-7~10-6mol/L)则对细胞有促增殖作用,最高促增殖浓度为1.0×10-7mol/L。DNA ladder和荧光显微镜可观察到高浓度白藜芦醇作用后细胞典型的凋亡形态。免疫组化结果显示低浓度白藜芦醇作用后,细胞核内PCNA表达明显增加(P〈0.05)。结论:高、低浓度的白藜芦醇对MCF-7细胞分别表现为诱导凋亡和促增殖作用,呈现出植物雌激素对MCF-7细胞典型的双向调节作用。
Objective:Our goals were to examine the phytoestrogen-like effect of Resveratrol(RES) using in vitro tests.Methods:Effects of RES on proliferation of MCF-7 cells were detected by MTT test.The fluorescent microscopes by the staining of Hoechst 33342 and DNA gel electrophoresis analysis were used to determine the effects of MCF-7 cells by RES.Immunohistochemistry was used to assess PCNA expression.Results:A MTT test and growth curve showed that the proliferation of MCF-7 cells was inhibited by a high concentration of RES,and that its IC50 was 8.70×10-5mol/L.However,RES induced the proliferation of MCF-7 cells at 10-7~10-6mol/L,and resulted in a peak proliferation at 1.0×10-7mol/L.After the cells were treated with high concentrations RES,morphological characteristic of apoptosis was detected.Agarose gel electrophoresis showed typical DNA ladder.The expression of PCNA was increased after treated with low concentrations of RES.Conclusions:These results suggest that RES had a dual regulatory effect on MCF-7 cells.
出处
《现代生物医学进展》
CAS
2011年第12期2254-2257,共4页
Progress in Modern Biomedicine
基金
武警医学院青年基金项目(WYQ201003)