一株原生质体融合菌株发酵菊芋生产酒精的工艺条件优化

Optimization of Alcoholic Fermentation of a Protoplast Fusion Strain from Jerusalem Artichoke

将试验筛选的具有分泌菊粉酶和产乙醇性能的融合菌株R8进行酒精发酵条件优化，经过6个单因素试验和正交试验，得到的优化条件为：160g／LJAE（菊芋提取液），25g／LBE（牛肉膏），8g／LNH4H2PQ4，0．1g／LZnCl2，pH6．0，30℃，转速180r／min。最终酒精得率为46．30％，原料糖分利用率能达到84．96％。

The fusant strain called R8 which can secrete inulinase and produce alcohol was chosen to optimize the alcoholic fermentation in order to get high-yielding alcohol fermentation conditions. The optimum condition confmned through six single factor experiments and the orthogonal test was list as following： 160g/L JAE （Jerusalem artichoke extract）, 25g/L BE （beef extract）, 8g/L NH4H2PO4, 0.1 g/L ZnC12, pH6.0, 30°E, 180r/min. Finally, the yield of alcohol is 46.30% and the rate of raw material utilization is 84.96%.