自噬相关基因Beclin1和MAPLC3在急性白血病骨髓单个核细胞中的表达及其意义

Expression of Autophagy Related Gene Beclin1and MAPLC3 in Bone Marrow Mononuclear Cells Isolated from Acute Leukemia Patients and Its Significance

本研究旨在检测人急性白血病(AL)骨髓单个核细胞中自噬相关基因Beclin1和微管相关蛋白质轻链3(MAPLC3)的表达,并探讨其意义。采用透射电子显微镜(TEM)和RT-PCR方法检测初治、复发难治、完全缓解(CR)的急性白血病患者和正常人骨髓单个核细胞(BMMNC)中的自噬活性以及Beclin1、MAPLC3mRNA的表达。结果表明:急性白血病初治组骨髓单个核细胞自噬活性、Beclin1和MAPLC3mRNA表达分别为80%、0.68±0.18、0.24±0.06;在难治复发组分别为100%、0.79±0.09、0.30±0.07;在完全缓解组分别为40%、0.52±0.15、0.16±0.04;在正常对照组分别为20%、0.57±0.13、0.16±0.05。初治组和难治复发组自噬活性和Beclin1、MAPLC3mRNA的相对表达量均高于正常对照组,差异有统计学意义(p<0.05)。完全缓解组和正常对照组之间比较差异无统计学意义(p>0.05)。结论 :初治组急性白血病骨髓单个核细胞中自噬活性和自噬相关基因Beclin1、MAPLC3mRNA的表达均上调,在难治复发组自噬活性和自噬相关基因Beclin1、MAPLC3mRNA的表达上调尤为明显,这可能与急性白血病的发生、发展及其耐药相关。

This study was purposed to detect the expression of autophagy-related gene Beclinl and microtubule-associated protein 1 light chain 3 （MAPLC3） in bone marrow mononuclear cells （BMMNC） isolated from acute leukemia （AL） patients, and to explore its significance. Transmission electron microscopy and RT-PCR were used to detect the autophagy activity and the expression level of Beclinl and MAPLC3 mRNA in BMMNC isolated from 27 AL patients with de novo, refractory or relapse AL and completely remission and 31 normal persons respectively. The results showed that autophagy activity and expression levels of Beclinl and MAPLC3 rnRNA in BMMNC from de novo AL patients were 80%, 0.68 ±0.18, 0.24±0.06, respectively; those in BMMNC from refractory or relapse AL patients were 100%, 0.79 ± 0.09, 0.30 ± 0.07, respectively ; those in BMMNC from CR patients were 40%, 0.52 ± 0.15, 0.16 ± 0.04, respectively, while those in BMMNC from normal persons were 20%, 0.57 ±0. 13, 0. 16 ±0.05, respectively. The autophagic activity and expression levels of Beclinl and MAPLC3 mRNA in de novo and refractory or relapse AL patients were higher than those in normal persons, with statistical significance （p 〈0. 05）, while the comparison between CR patients and normal control showed no statistical difference（p 〉0.05 ）. It is concluded that autophagy activity and Beclinl and MAPLC3 mRNA expression level of in de novo and refractory or relapse patients are higher than those in normal control, and the up- regulation of antophagy activity and expression of Beclinl and MAPLC3 mRNA in refractory or relapse patients is especially significant. This may be related to the genesis, development and drug resistance of AL.