鼻咽癌血清蛋白指纹图谱的初步研究

A pilot investigation on serum protein fingerprinting of nasopharyngeal carcinoma

目的比较鼻咽癌患者和对照组之间以及不同病理类型鼻咽癌之间的血清蛋白指纹图谱差异，为建立鼻咽癌血清蛋白组学诊断模型奠定初步基础。方法采集经病理证实的鼻咽癌患者血清41例和对照者血清20例。采用表面增强激光解吸电离飞行时间质谱测定技术获得血清蛋白质谱即血清蛋白指纹图谱，应用专门软件比较鼻咽癌组和对照组的血清蛋白质谱，筛选出差异蛋白，并检查分化型非角化性癌，未分化型非角化性癌和未分化癌三种不同病理类型鼻咽癌血清蛋白相对含量的差异。结果鼻咽癌组质荷比15931．654等9处血清蛋白的相对含量显著高于对照组（P值均〈0．001），表现为组合性差异蛋白峰；三种病理类型鼻咽癌不同分子质量的血清蛋白相对含量比较差异无统计学意义（P〉0．05）。结论应用表面增强激光解吸电离飞行时间质谱技术可筛选出鼻咽癌相关血清蛋白及其蛋白指纹图谱，有望为鼻咽癌的临床筛查和早期诊断建立血清蛋白组学模型。

Objective To establish a preliminary foundation for developing a serum proteomics diagnostic model of nasopharyngeal carcinoma （NPC） by comparing the differences in serum protein fingerprints between patients with NPC and healthy subjects and between different types of NPC. Methods The serum samples of 41 patients with different types of NPC and 20 healthy subjects were collected. Surface-enhanced laser desorption ionization time-of-flight mass spectrometry （SELDI-TOF-MS）were used to detect the blood samples to obtain serum protein mass spectrum, i. e. serum protein fingerprinting. Biomarker Wizard and Biomarker Patterns system software were used to compare the differences in serum protein mass spectrum between NPC patients and healthy subjects and between different types of NPC, and to screen out the NPC-related serum proteins. Results Compared with the healthy control, NPC patients emerged 9 very prominent protein peaks（P 〈 0. 001 ） , with the combined differential peaks. No significant difference was found in relative amount of serum proteins with different molecular mass between different types of NPC （ P 〉 0.05 ）. Conclusions The serum marker proteins and specific protein fingerprints of NPC can be screened out by SELD1-TOF-MS, which could be used to develop a serum proteomics model for clinical screening and early diagnosis of NPC.