2型糖尿病骨质疏松大鼠骨重建研究

Research of bone remodeling in osteoporosis rats with type 2 diabetes mellitus

目的通过建立2型糖尿病骨质疏松大鼠模型,探讨其骨重建特点。方法 雌性Wistar大鼠分为假手术组(NS组);去卵巢组(NOVX组);2型糖尿病假手术组(DS组)和2型糖尿病去卵巢组(DOVX组)。糖尿病组大鼠造模成功后行双侧卵巢切除术,0、4、8和12周测血糖(FPG)、胰岛素(FINS)、雌激素(E2)及血清骨特异性碱性磷酸酶(B-ALP)和抗酒石酸酸性磷酸酶(TRACP5b),同期测定骨密度(BMD)并行成骨和破骨细胞培养。结果 0周时DOVX组的血糖(13.1±4.9)mmol/L明显高于NS组的(4.4±0.6)mmol/L(P<0.05)。4周时DOVX组的BMD为(0.073±0.012)g/cm2明显低于NS组的(0.098±0.016)g/cm2(P<0.05)。4、8和12周时DOVX组的B-ALP与NS组均无差异,而4和8周时DOVX组的TRACP5b明显高于NS组(P<0.05)。4、8和12周时DOVX组的成骨细胞活力与NS组均无差异,而DOVX组的破骨细胞计数明显高于NS组(P<0.05)。结论 2型糖尿病骨质疏松大鼠骨重建特点为骨吸收活跃而骨形成相对减慢。

Objective To characterize bone remodeling by establishing type 2 diabetic osteoporosis rat model.Methods Female Wistar rats were randomly divided into sham operation group（NS）;bilateral ovariectomy group（NOVX）;diabetic sham operation group（DS） and diabetic ovariectomized group（DOVX）.Ovariectomy was performed in both control and diabetic rats after type 2 diabetic models had been made.Venous blood samples were collected at 0、4、 8 and 12wks after ovariectomy,fasting plasma glucose（FPG）、fasting insulin（FINS）、estrogen（E2） and specific bone turnover markers such as alkaline phosphatase（B-ALP）,tartrate-resistant acid phosphatase（TRACP5b） were all measured.BMD were measured,osteoblast and osteoclast cell were isolated and cultured from rats of all groups.Results At 0wks,FPG had significantly increased when DOVX′ 13.1±4.9 mmol/L compared with NS′ 4.4±0.6 mmol/L（all P0.05）.at 4wks,BMD had significantly decreased when DOVX′ 0.073±0.012 g/cm2 compared with DS′ 0.098±0.016 g/cm2（P0.05）.at 4、8 and 12wks,there was no differences in plasma B-ALP levels when DOVX rats compared with NS rats.at 4 and 8wks,compared with NS rats,DOVX rats had significantly increased plasma TRACP5b concentration（all P0.05）.For the activity of osteoblast,there wasno differences when DOVX compared with NS,NOVX and DS group.osteoclast numbers was significantly increased in DOVX rats than those in NS rats（P0.05）.Conclusion There was active bone resorption and relatively slow bone formation in osteoporosis rats with type 2 diabetes mellitus.