白藜芦醇对人黑色素瘤细胞增殖及凋亡的影响

Effects of resveratrol on proliferation and apoptosis in human melanoma cells

目的研究白藜芦醇(resveratrol,Res)对人黑色素瘤细胞增殖及凋亡的影响。方法 MTT法检测不同浓度(10、20、40、80、160μmol.L-1)Res对人黑色素瘤细胞株Mel-RM和MM200增殖的抑制作用,溴化丙啶(propidium iodide,PI)单染进行流式细胞仪分析,检测Res对黑色素瘤细胞凋亡的影响。通过JC-1染色法检测Mel-RM和MM200细胞线粒体膜电位(mitochondrial membrane potential,△Ψm)。试剂盒检测Caspase-3的活性变化。结果随着Res的浓度的增加,对人黑色素瘤细胞株的增殖产生明显的抑制作用,80、160μmol.L-1的Res可诱导Mel-RM和MM200细胞出现明显的凋亡,JC-1染色结果显示,Res处理后Mel-RM和MM200线粒体膜电位分别降低51.0%和68.8%。Caspase-3活性检测结果显示Res对Caspase-3具有激活作用。结论 Res具有抑制黑色素瘤细胞增殖诱导其凋亡的作用,其机制可能与降低细胞线粒体膜电位及激活Caspase-3有关。

Aim To investigate the effect of resveratrol（Res） on the proliferation and apoptosis in human melanoma cells.Methods Mel-RM and MM200 cells were treated with different concentrations of Res.Cell viability was measured using the MTT assay.Apoptosis induced by Res was examined using the propidium iodide（PI） staining in flow cytometry.Mitochondrial membrane potential（△Ψm） in Mel-RM and MM200 cells was detected by JC-1 staining.Activation of Caspase-3 was detected by special kit.Results Cell viability was inhibited by Res with the increasing concentration.80,160 μmol·L-1Res could induces obvious apoptosis in melanoma Mel-RM and MM200 cells.JC-1 staining results showed that Res could decrease the mitochondrial membrane potential in Mel-RM and MM200 cells.It also induced the activation of Caspases-3.Conclusions Res can inhibit the proliferation and induce apoptosis in melanoma Mel-RM and MM200 cells.The effect might be associated with the reduction of △Ψm and the activation of Caspase-3.