新乡红豆杉中多种紫杉烷类化合物的反相高效液相色谱分析

Analysis of taxanes in Chinese yew planted in Xinxiang by reverse-phase high-performance liquid chromatography

采用反相高效液相色谱法(RP-HPLC)研究了新乡种植红豆杉树皮中的多种紫杉烷类化合物.结果表明,新乡种植红豆杉树皮中的10-去乙酰巴卡丁Ⅲ、巴卡丁Ⅲ、7-木糖-10-去乙酰基三尖杉宁碱、7-木糖-10-去乙酰基紫杉醇、7-木糖-10-去乙酰基紫杉醇C、10-去乙酰基紫杉醇、三尖杉宁碱和紫杉醇的平均含量(质量分数,n=4)分别为:0.011%、0.012%、0.011%、0.053%、0.012%、0.031%、0.036%、0.040%.

Various taxanes in the leafs of Chinese yew planted in Xinxiang were analyzed by means of reverse-phase high-performance liquid chromatography（RP-HPLC）.It was found that the content（mass percent,n=4） of various taxanes including 10-deacetyl baccatin III,baccatinⅢ,7-xylosyl-10-deacetyl cephalomannine,7-xylosyl-10-deacetyl taxol,7-xylosyl-10-deacetyl taxol C,10-deacetyl taxol,cephalomannine,and taxol in the leafs of Chinese yew planted in Xinxiang was 0.011%,0.012%,0.011%,0.053%,0.012%,0.031%,0.036%,and 0.040%.