摘要
采用高效液相色谱法,用氨基柱(250×4.6 mm)对VC及脱氢VC进行分离测定。流动相乙腈和三氟乙酸的比例为85∶15,脱氢VC和VC的保留时间分别为3.93 min和4.45 min。
For the simultaneous determination of dehydroascorbic acid and ascorbic acid,the sample is separated by using amino analysis column（250×4.6 mm）,and a mobile phase consisting of acetonitrile / 0.1% trifluoroacetic（85∶15）.The retention times of dehydroascorbic acid and ascorbic acid are 3.93 min and 4.45 min respectively.
出处
《河北化工》
2011年第6期31-32,43,共3页
Hebei Chemical Industry
