94例缺血性脑卒中患者HLA基因表达分析

Expression of human leukocyte antigen gene in 94 patients with cerebral infarction

目的探讨人类白细胞抗原（HLA）基因遗传与缺血性脑卒中发病的关联。方法采用聚合酶链反应-直接测序分型技术（PCR-SBT）对广东医学院附属南山医院2008年收治的94例缺血性脑卒中患者和同期122例正常对照者进行HM-A、B、DRB1位点的等位基因分型。结果缺血性脑卒中患者表达HLA-A位点16个等位基因．HLA-B位点32个等位基因．HLM—DRB1位点25个等位基因。患者组HLA-A＊1102基因频率较对照组明显降低，HLA-A＊1102与缺血性脑卒中呈负相关（RR=0．06，P=0．019）。结论HLA-A＊1102与缺血性脑卒中呈易感负相关，提示HLA等位基因与缺血性脑卒中的发生存在遗传免疫关联，对该病具有临床预测意义。

Objective To discuss the relationship between human leukocyte antigen （HLA） gene heredity and morbidity of cerebral infarction by a random survey on the allele expression of HLA-A, B and DRB 1 seats of patients with cerebral infarction. Methods The genotypes of HLA-A, B and DRB 1 alleles in 94 patients with cerebral infarction and 122 healthy blood donors were detected by polymerase chain reaction-sequencing based typing （PCR-SBT） method. Results Sixteen alleles in HLA-A locus, 32 alleles in HLA -B locus and 25 alleles in HLA -DRB1 locus expressed themselves in these patients with cerebral infarction. The gene frequency of HLA -A＇1102 in patients was lower than that in healthy controls, and negative association was found between HLA -A＊1102 allele and cerebral infarction （RR=0.06,P=0.019）. Conclusion The research reveals susceptibility association of HLA -A＊1102 with patients having cerebral infarction, displaying close genetic immunity correlation between HLA alleles and pathogenesis of cerebral infarction. So, the research in this paper is useful in the clinical prediction of this disease.