汉坦病毒感染C57BL/6小鼠组织中特异性抗原的检测

Detection of the specific antigens in the tissues of the C57BL/6 mouses infected with hantavirus

目的通过检测汉坦病毒感染C57BL/6小鼠组织中特异性病毒抗原,以建立汉坦病毒感染动物的评价体系。方法将汉坦病毒陈株按照原病毒液、10-1、10-2三个滴度经肌肉注射感染C57BL/6小鼠,在感染后的第3、6、9、12、15天,分别取小鼠的心、肝、脾、肺、肾、脑等组织研磨后制成病毒悬液,以ELISA法检测各组织中的病毒特异性抗原。结果 C57BL/6小鼠感染汉坦病毒后短期内在其肝脏和脾脏可以检测到特异性抗原,随着时间的延长,这些抗原逐步消失。结论上述结果为建立汉坦病毒感染动物的评价体系提供了一种参考。

Objective To establish a system to evaluate the animals infected with hantavirus.Methods C57BL/6 mice were intramuscular infected with various doses of hantavirus（10-1 to 10-2 dilution of stock solution）.Viral antigen in the mouse tissues was detected by ELISA method at day-3,6,9,12,and 15 after infection.Results Viral antigens could be detected from the liver and spleen samples early after infection.The expression level was reduced gradually.Conclusion The results provided some information about the animals infected with hantavirus.